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《JOURNAL OF MICROBIOLOGY》 1994-01
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STUDY ON REGERATIONAL CONDITIONS OF MONOKARYOTIC MYCELIUM 9101(12) OF LENTINUS EDODE

Zhang Changkai et al.(Depart of Microbiology Shandong Universivy, Jinan, 250100)  
Conditions for regenerating the protoplasts from monoaryotic mycelium 9101 (12) were defined. The protoplasts were cultivated in an MYG medium for five days and then regenerated in the hypertonic MYG medium containing 0. 6M MgSO4, thereby the regeneration frequency being raised to 5×10-5, Furthermore, the method developed by Knowlton et al. for isolating high-regeneration-frequency strains was for the first time applied to the edible mushroom Lent inns edodes, By using this method we obtained a regeneration strain of R3, the regeneration frequency of which is 2-3 tinies that of monokaryotic mycelium 9101 (12).
【CateGory Index】: S646.120.1
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1 Zhang Changkai Tang Shengrōng Du Jian Bai Yuqian Lu Qingda Li Cuimei;THE HYBRIDIZATION OF LENTINUS EDODES BASIDIOSPORE[J];山东大学学报(自然科学版);1987-04
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 Zhang Changkai et al.(Depart of Microbiology Shandong Universivy, Jinan, 250100);STUDY ON REGERATIONAL CONDITIONS OF MONOKARYOTIC MYCELIUM 9101(12) OF LENTINUS EDODE[J];微生物学杂志;1994-01
【Co-references】
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1 Lin Fucheng Liu Xiaohong Wang Hongkai Zhang Chulong ( 1Life Sciences College of Zhejiang University, 2Biotechnology Institute of Zhejiang University, Hangzhou 310029,China);Recent Research and Prospect on Taxol and Its Producing Fungi[J];微生物学报;2003-04
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6 Zhang Changkai et al.(Depart of Microbiology Shandong Universivy, Jinan, 250100);STUDY ON REGERATIONAL CONDITIONS OF MONOKARYOTIC MYCELIUM 9101(12) OF LENTINUS EDODE[J];微生物学杂志;1994-01
【Secondary References】
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1 LI Jing;LIN Xiongjie;LIN Dongmei;LU Guodong;LIN Zhanxi;China National Juncao Engineering Research Center;Juncao Institute,Fujian University of Agriculture and Forestry;Fruit Institute,Fujian Academy of Agricultural Science;;Establishment of Genetic Transformation System of Antrodia cinnamomea Based on PEG-CaCl_2 Mediated Method[J];贵州农业科学;2016-04
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7 PANG Chun-Mei ZHANG Liang SUN Chun-Yu JIANG Shi-Cui WANG Kang-Yu ZHANG Mei-Ping WANG Yi College of Life Sciences, Jilin Agricultural University, Changchun, Jilin 130118, China;Transformation of large DNA fragment of Panax ginseng into Ganoderma lucidum[J];菌物学报;2013-01
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9 Zhu Jian 1,2,Zhang Peng 1,2 (1 Mycological Research Center,Fujian Agriculture and Forestry University,Fuzhou 350002;2 College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002);Optimizing Conditions of Preparing Pleurotus nebrodensiss Protoplast[J];中国农学通报;2011-25
10 XU Xiuhong1,MENG Qi1,LIU Huajing1,LI liang2(1.College of Resources and Environmental Sciences,Northeast Agricultural University,Harbin 150030,China;2.Heilongjiang Academy of Agricultural Sciences,Daqing Heilongjiang 161090,China);Study on protoplast isoulation and regeneration and monokaryotization of Auricularia auricula[J];东北农业大学学报;2011-08
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