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《ACTA MICROBIOLOGICA SINICA》 1998-04
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ISOLATION, PURIFICATION AND IDENTIFICATION OF METALLTHIONEIN FROM STRAIN BD 101 OF SACCHAROMYCES CEREVISIAE

Lin Zhilan;Chang Limei (College of Life Sciences, Peking University, Beijing 100871)  
Selection of Saccharomyces cerevisiae BD101 having resistance to metal ions and producing metallothionein by inducer from Saccharomyces. These proteins were isolated and Purified by Sephadex G-50 and subsequent DEAE Sepharose CL-4B,then Sephadex G-25 for desalination. There were two iso-MTs for Cu-induced. The,molecular weightS were 7 kD and had 60 amino acids. Both Cu-MTs were rich in Cysteine (10%). 4 atom CU / 6 mole Cys / mole CU-MTs.
【Fund】: 国家自然科学基金!39370021
【CateGory Index】: TQ201.3
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