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《Acta Microbiologica Sinica》 2003-02
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Extraction and Purification of Soil Microbial Total DNA

Zhang Ruifu 1 Cao Hui 1,2 Cui Zhongli 1** Li Shunpeng 1 Fan Ben 1 ( 1Key Lab of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University,Nanjing 210095,China) ( 2Institute of Soil Science, the Chinese Academy of Sciences, Nanjing 210008, China)  
A method of extracting soil total DNA was developed, and it can extract DNA from G + bacteria. The soil total DNA was extracted from 9 different soils,the DNA yeild was from 3 30μg to 13 41μg. The crude soil DNA was purified by dialysis bag, the recovery rate is 65 34%。The extracted rate in the 9 soils was 60 51%~93 45%. The traget gene could be amplified from the soil which was added 362 target cell/g.dry soil.
【Fund】: 中国科学院南京土壤研究所物质循环开放实验室开放课题 (2 0 2 5 1 0 2 );; 红壤站开放课题 (2 0 0 1 K 0 1 )资助项目~~
【CateGory Index】: S154.3
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