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Method of separate culture of primary neurons using glial feeder layer

Bai Xuetao Institute of Environmental Health Monitoring, Chinese Academy of Preventive Medicine, Beijing 100021, China  
In order to obtain primarily cultured neurons that can live longer and are healthy, and easy to be isolated and purified, the glial feeder layers are used for culturing the primary neurons separated from the glial cells. The result shows that this method is reliable and easy to be handled. The cultured neurons are pure, and healthy. They can survive longer and have potential to be utilized in many relevant studies.
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