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《Journal of Hygiene Research》 2005-01
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Influence of resistant starch on colon flora of mice

He Mei,Hong Jie,Yang Yue-xin,Shen Xiang,et al. National Institute of Nutrition and Food Safety,Chinese Center for Disease Control and Prevention,Beijing 100050,China  
Objective To investigate the effect of resistant starch (RS_2 and RS_3) intake on colon flora of mice.Methods 32 mice were divided into four groups (8 mice/group) according to their weight randomly.Four groups of mice were given basic feed (containing only corn starch and no RS),containing 6% RS_2 feed,containing 12% RS_2 feed,and containing 6% RS_3 feed,respectively.Feces were collected on the 1st and 29th day of the experiment,and pH of feces were measured and five feces flora (enterobacter,bifidobacteria,lactobacillus,bacteroid,and enterococcus) were detected with culture medium method.All the mice were killed on the 29th day to get caecum content.The pH of caecum content were measured and short chain fatty acid (SCFA) were detected with gas chromatography.Results RS_2 and RS_3 could all increase feces bifidobacteria (P0.05) and decrease enterobacteria (P0.05).RS_2 could increase SCFA of ceacum content,and 12% RS_2 group is more than control group(P0.01),and also more than 6% RS_2 group (P0.05).RS could also decrease pH of feces and ceacum content of mice,and the effect of RS_3 is more than RS_2. Conclusion Resistant starch could improve colon flora,increase its fermentable production SCFA,decrease caecum and feces pH,and then improve health.
【Fund】: 国家自然科学基金资助项目(No.30070657)
【CateGory Index】: R151.3
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