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《Journal of Hygiene Research》 2005-02
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Identification of the related gene of mouse tolerant to exposure to perfluoroisobutylene by DDRT-PCR

Tang Zhi-hua, Rong Shu, Ding Shu-biao Nanjing Military Medical Institute, Nanjing\ 210002, China  
Objective\ The mice surviving acute exposure to low do se of perfluorois obutylene (PFIB) could tolerant to lethal dose of PFIB. Discovering the gene rel ated with the tolerance would be significant to find preventive agents for PFIB. Methods By static exposure to PFIB for 3 times,the survival ani mals could tolera te to lethal dose of PFIB.Three anchoring primers and 4 arbitary primers were ap plied in differential display reverse transcription PCR(DDRT-PCR) of lung tissu e s and the products were analyzed by sequencing PAGE and silver staining.The diff erential DNA fragments were cloned and sequenced.Results There were obvious diff erences of gene expresion between control and tolerant animals.Three expressed s equence tags(EST) have been cloned and sequenced,which were homologous to some m ouse gene in Genbank. Conclusion Specific gene up-regulation m inght be the cause of tolerance to PFIB, and they are very important to clarity the tolerant mecha nisms to PFIB.
【Fund】: 全军“十五”指令性课题资助项目(No.01L077)
【CateGory Index】: R114
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【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Hu 1,2,WANG He-mei 1,ZHAO Jian 1,DING Ri-gao 1,HUA Zi-chun 2;Pathogenesis of perfluoroisobutylene inhalation-induced acute lung injury[J];中国职业医学;2013-03
【Co-references】
Chinese Journal Full-text Database 8 Hits
1 MENG Ge,WANG He-mei,ZHAO Jian,DING Ri-gao,ZHANG Xian-cheng,HUANG Chun-Qian (Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China);Long-term effect of a single perfluoroisobutylene exposure induced acute lung injury in mice[J];中国药理学与毒理学杂志;2011-06
2 WANG He-mei,HUANG Chun-qian,ZHANG Xian-cheng,QU Wen-sheng,SUN Xiao-hong, DING Ri-gao,RUAN Jin-xiu(National Beijing Center for Drug Safety Evaluation and Research,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China);The time-course effect of perfluoroisobutylene inhalation on endogenous nitric oxide and its synthases activity in rats[J];军事医学科学院院刊;2010-06
3 ZHANG Xian-cheng~1,XU Jin~2,LIN Juan~3,SHAO Zhi-hua~2,HUANG Chun-qian~1,SUN Xiao-hong~1,ZHANG Tian-hong~1,ZHAO Jian~1,DING Ri-gao~1(1.Institute of Toxicology and Pharmacology,Academy of Military Medical Science,Beijing 100850,China;2.Zhejiang Quhua Hospital,Quzhou 324004,China;3.The Municipal Hospital of Yantai City,Yantai 264000,China);Effects of 2-PAM on acute lung injury induced by PFIB inhalation in mice[J];中国工业医学杂志;2006-05
4 ZHAO Min,CHEN Jia-bin,WANG He-mei,et al(Beijing Institute of Pharmacology and Toxicology,Beijing 100850,China);The effect of alveolar macrophage inhibition in perfluoroisobutylene-induced acute lung injury[J];中国职业医学;2006-03
5 ZHAO Jian,ZHANG Tian-hong,DING Ri-gao,ZHANG Xian-cheng,HUANG Chun-qian,SUN Xiao-hong(Institute of Pharmacology and Toxicology,Academy of Military Medical Science,Beijing 100850,China);Effects of inhaled perfluoroisobutylene on sulfhydryls in the lung tissues of mice[J];中国工业医学杂志;2005-05
6 Tang Zhi-hua, Rong Shu, Ding Shu-biao Nanjing Military Medical Institute, Nanjing\ 210002, China;Identification of the related gene of mouse tolerant to exposure to perfluoroisobutylene by DDRT-PCR[J];卫生研究;2005-02
7 ZHAO Jian, DING Ri-gao, RUAN Jin-xiu, et al (Institute of Pharmacology and Toxicology, Academy of Military Medical Science, Beijing 100850, China);Effects of tetracycline hydrochloride on acute lung injury induced by perfluoroisobutylene inhalation in mice[J];中国职业医学;2005-01
8 ZhangXiancheng,WangWenzhu,PengShuangqing,etal.DepartmentofMilitaryToxicologyofInstituteofPharmacologyandToxicology,AcademyofMilitaryMedicalSciences.Beijing100850;Studiesontheprotectiveeffectsofdifferentabsorbentsagainstperfluoroisobutylene[J];中国工业医学杂志;1999-03
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 Yang Limei;Zhao Jian;Wang Haitao;Xu Xingxing;JIiao Yumei;Ding Rigao;Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences;;The protective effect of N-acetylcysteine on acute lung injury induced by PFIB inhalation[J];中华劳动卫生职业病杂志;2017-07
2 Wang Lijun;Wang Hao;Jiang Hong;Qian Jun;Haojian;Department of Respiratory Medicine,Tongling People's Hospital;;The oxidative stress effect of protease inhibitor on PFIB-inhalated acute lung injury in rats[J];国际呼吸杂志;2016-17
3 WANG Yan-lin;WANG Hu;ZHAO Jian;HUANG Chun-qian;SUN Xiao-hong;DING Ri-gao;WANG He-mei;Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences;;Study on preventive and therapeutic effects of losartan potassium on perfluoroisobutylene-induced acute lung injury in rats[J];中国职业医学;2014-03
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