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《Journal of Hygiene Research》 2009-06
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Studies on performance standard for hemolytic toxins in harmful bloom algae

PENG Yinghui,LIU Jiesheng,LI Hongye,HUANG Zhanchao,LI Xiuqin,YANG Weidong School of Life Science and Technology,Jinan University,Guangzhou 510632,China  
Objective To explore a performance standard for hemolytic toxins in harmful bloom algae.Methods Using Chattonella marina as hemolytic substances producing organism,methods and conditions were compared and optimized including cell breakage,distillation temperature,blood origin and storage of algal pellets in extraction and activity determination of hemolytic toxins.Results The hemolytic activity of C.marina broken by supersonic method was 288.23 HU/L,higher than that by freezing-thawing method(94.89 HU/L),suggesting that supersonic method could be more optimal to break microalgal cells.When the supersonic treatment times were 5,10,20 and 30min,the hemolytic activities were 80.57,157.45,288.23 and 279.17HU/L,respectively,indicating that 20min of supersonic treatment was suitable.When the distillation temperature were 40,60 and 80℃,the hemolytic activities were 288.23,124.97 and 120.68HU/L,respectively,meaning that high distillation temperature in extraction of hemolytic substances lowed the hemolytic activities of samples.Bloods from various animals such as human,fish,rat and rabbit exhibited different sensitivity to the hemolytic toxins,of which rabbit erythrocyte was the most sensitive.The hemolytic activities to human,fish,rat and rabbit were 244.98,288.23,266.35 and 195.47HU/L,respectively.The storage of algal pellets for 3 days at the temperature of 0℃ did not reveal a significant loss in hemolytic activity,while significant losses were observed at the temperature of 20℃ or-20℃only after one day.Conclusion Supersonic method could be more optimal to break cell in comparison with freeze-thaw method.Optimal conditions for broken algal cells by supersonic method were 200 W for 20min at the temperature of 4℃.The distillation temperature in extraction of hemolytic substances should be maintained under the temperature of 40℃.The rabbit erythrocyte could be the most optimal blood to detect hemolytic activity due to its high sensitivity.The algal pellets could be kept at the temperature of 0℃ for 3 days before determination of activity.
【Fund】: 国家自然科学基金-广东省联合基金重点项目(No.U0733006);; 国家自然科学基金(No.30970502)
【CateGory Index】: X55
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【References】
Chinese Journal Full-text Database 6 Hits
1 CAO Jie-ru;HUAN Qing-liu;WU Ni;JIANG Tian-jiu;Research Center of Harmful Algae and Marine Biology;Key Laboratory of Eutrophic Water and Harmful Algae Blooms Control and Mitigation,Guangdong High Education Office,Jinan University;;Effects of temperature,light intensity and nutrient condition on the growth and hemolytic activity of six species of typical ichthyotoxic algae[J];海洋环境科学;2015-03
2 WU Ni,JIANG Tian-Jiu*,JIANG Tao(Research Center for Harmful Algae and Aquatic Environment,Jinan University,Guangzhou 510632,China);Analyses of Hemolytic Toxin from Ichthyotoxic Phytoplankton Chattonella marina(Hong Kong Strain) by High Performance Liquid Chromatography[J];分析化学;2012-08
3 LIU Tingting,SHI Zhanpeng,LI Hongye,LIU Jiesheng,YANG Weidong Department of Biotechnology,Jinan University,Guangdong Province's Higher Education Key Laboratory of Eutrophication and Red Tid Control,Guangzhou 510632,China;Cytotoxicity of Karenia mikimotoi extracts[J];卫生研究;2012-03
4 JIANG Tao a,TENG De-qiang a,JIANG Tian-jiu a,L Song-hui a,YANG Wei-dong b(a.Research Center for Harmful Algae and Aquatic Environment;b.Department of Biology,Jinan University,Guangzhou 510632,China);Advances in Hemolytic Toxins of Marine Microalgae[J];热带亚热带植物学报;2012-03
5 JIANG Tao1,WU Ni1,ZHONG Yan2,JIANG Tian-jiu1,2(1 Research Center for Harmful Algae and Aquatic Environment,Jinan University,Guangzhou 510632,China;2 College of Life Science,South China Normal University,Guangzhou 510630,China);Study on the hemolytic activity of Chattonella ovata[J];安全与环境学报;2012-02
6 SHI Zhanpeng,LIU Jiesheng,LI Hongye,CUI Weimin,YANG Weidong Department of Biotechnology,Jinan University,Guangdong Province's Higher Education Key Laboratory of Eutrophication and Red Tid Control,Guangzhou 510632,China;Effects of some membrane lipids on the hemolysis induced by hemolytic toxin from Karenia mikimotoi[J];卫生研究;2011-03
【Citations】
Chinese Journal Full-text Database 9 Hits
1 ZHANG Wen1,JIANG Tian-jiu1,2,WANG Rui1 1. Environment Engineering department, Jinan university, Guangzhou 510632, China; 2. Researcher Center of HABs and Water Environmental Science, Guangzhou 510632, China;The extraction and separation of hemolytic toxin from Chattonella marina HongKong strain[J];生态科学;2008-06
2 YUAN Mei-Ling,WANG Zhao-Hui,LI You-FuInstitute of Hydrobidogy,Jinan University,Guangzhou 510632,China;Effects of nitrogen and phosphorus limitation on the growth of Chattonella marina[J];生态学报;2008-01
3 GUO Jin, YANG Weidong, LIU Jiesheng*,FAN Zhenhua Department of Biotechnology, Jinan University, Guangzhou 510632;Effects of salinity, temperature and light intensity on the growth and toxin production of Phaeocystis globosa[J];环境科学学报;2007-08
4 LIU Jie-sheng1,PENG Xi-chun2,YANG Wei-dong1(1.College of Life Science and Technology,Jinan University,Guangzhou 510632,China;2.College of Food Science and Technology,Jinan University,Guangzhou 510632,China);AFM observation of hemolytic extracts from Phaeocystis globosa on rabbit erythrocytes[J];热带海洋学报;2007-02
5 LI Tao, LIU Sheng, HUANG Liang-min, XU Zhan-zhou, LI Kai-zhi, SU Qiang, SONG Xing-yu, ZHANG Jian-lin(South China Sea Institute of Oceanology, The Chinese Academy of Sciences, Guangzhou 510301, China);STUDIES ON PHYTOPLANKTON COMMUNITY CHANGE AT DAYA BAY DURING A RED TIDE[J];热带海洋学报;2005-03
6 PENG Xi-chun1, YANG Wei-dong2, LIU Jie-sheng2*,PENG Zhi-ying1, DENG Rui-xia2, JIANG Tian-jiu2 (1. Food and Biological Engineering College, South China University of Technology, Guangzhou 510640, China; 2. College of Life Sciences and Technology, Jinan University, Guangzhou 510632, China);Extraction of Haemolytic Substances from Alga Phaeocystis globosa[J];热带亚热带植物学报;2005-01
7 YIN Yi wei, WANG Zhao hui, JIANG Tian jiu, LUO Yu min, XIE Long chu (Institute of Hydrobiology, Jinan University, Guangzhou 510632, China);Toxic effects of red tide toxins on fishes[J];海洋环境科学;2000-04
8 Qi Yuzao; Huang Changjiang(Institute of Hydrobiology, Jinan University, Guangzhou 510632);THE CAUSATIVE MECHANISM OF CHATONELLA MARINA BLOOM IN DAPENG BAY, THE SOUTH CHINA SEA[J];海洋与湖沼;1997-04
9 He Jiawan; Chen Minghui and He Zhenrong(Institute of hydrobiology, The Chinese Academy of Sciences, Wuhan 430072);ISOLATION AND CHARACTERIZATION OF TOXINS FROM THE PHYTOFLAGELLATE PRYMNESIUM PARVUM[J];水生生物学报;1996-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XU Shan-nan;LIN Hua-jian;GONG Yu-yan;LI Chun-hou;CHEN Zuo-zhi;Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization,Ministry of Agriculture,Key Laboratory of Fishery Ecology and Environment,Guangdong Province,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;;Ecological characteristics of phytoplankton community structure in Northwest Hainan coastal areas[J];海洋环境科学;2015-05
2 PANG Yong;NIE Rui;OU Lin-jian;LV Song-hui;Hainan Academy of Oceanographic and Fishery Sciences;Research Center for Harmful Algae and Marine Biology,Jinan University;Marine Monitoring and Forecasting Center of Hainan Province;;The study on alkaline phosphatase activity( APA) of phytoplanktons in the bloom of Mesodinium rubrum based on ELF@97[J];海洋环境科学;2015-05
3 MENG Shunlong;QIU Liping;WANG Jing;HU Gengdong;QU Jianhong;FAN Limin;SONG Chao;WU Wei;CHEN Jiazhang;BING Xuwen;Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture; Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences;Wuxi Fishery College, Nanjing Agricultural University;;Effect of Light Intensity on Growth and Competition between Chlorella Vulgaris and Anabaena[J];生态环境学报;2015-10
4 YANG Qiu-chan;ZHAO Ling;YIN Ping-he;TAN Shuo;SHU Wan-jiao;HOU Shao-ling;Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes,School of Environment,Jinan University;College of Life Science and Technology,Jinan University;;Effects of Algicidal Substance on Phaeocystis globosa and Its Fatty Acids by the Simulation Experiment[J];环境科学;2015-09
5 HE En-ye;WANG Dan;HUANG Li;LIU Gui-mei;National Marine Environmental Forecast Center;;Impact of the West Pacific subtropical high on the red tide in the China seas[J];海洋预报;2015-04
6 HU Cui-lin;JIN Hai-wei;LI Zhen-hua;Marine Fisheries Research Institute of Zhejiang Province, Scientific Observing and Experimental Station of Fishery Resources for Key Fishing Grounds,MOA, Key Laboratory of Sustainable Utilization of Technology Research for Fisheries Resources of Zhejiang Province;Fishery School of Zhejing Ocean University;;Research Advance of Noctiluca scintillans[J];浙江海洋学院学报(自然科学版);2015-04
7 CHEN Bao-hong;XIE Er-yi;GAO Ya-hui;JI Wei-dong;ZHOU Qiu-lin;Third Institute of Oceanography,State Oceanic Administration;Third Institute of Oceanography,State Oceanic Administration,Xiamen 361005,China;School of Life Science of Xiamen University;;Toxic effects of red tide caused by Karenia mikimotoi on marine organisms[J];福建水产;2015-03
8 CAO Jie-ru;HUAN Qing-liu;WU Ni;JIANG Tian-jiu;Research Center of Harmful Algae and Marine Biology;Key Laboratory of Eutrophic Water and Harmful Algae Blooms Control and Mitigation,Guangdong High Education Office,Jinan University;;Effects of temperature,light intensity and nutrient condition on the growth and hemolytic activity of six species of typical ichthyotoxic algae[J];海洋环境科学;2015-03
9 Cai-rang-zhuo-ma;ZHAO Yun-tao;ZHANG Chao-hua;QIN Xiao-ming;ZHENG Hui-na;CAO Wen-hong;College of Food Science and Technology, Guangdong Ocean University, Guangdong Ocean University;Biochemistry Center, Guangdong Ocean University;Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Ocean University;Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University;National Research and Development Branch Center for Shellfish Processing (Zhanjiang) , Guangdong Ocean University;;Preliminary Research of Geographical Origin Traceability of Crassostrea hongkongensisbased on Inorganic Elements Analysis[J];广东海洋大学学报;2015-03
10 PANG Yong;NIE Rui;LU Songhui;Hainan Academy of Oceanographic and Fishery Sciences;Research Center for Harmful Algae and Marine Biology, Jinan University;Marine Monitoring and Forecasting Center of Hainan Province;;Preliminary Environmental Analysis of the Evolution of Cochlodinium geminatum Bloom in the Pearl River Estuary[J];生态环境学报;2015-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 GUO Hao;LIN Feng-ao;LIU Yong-jian;XU Dao-yan;LU Xing-wang;Environmental Science and Engineering College ,Dalian Maritime University;Key Laboratory for Ecological Environmental in Coastal Areas,State Oceanic Administration,National Environmental Monitoring Center;;High-incidenceHABs causative species in China Coastal Waters and the forewarning method based on the HABs Risk Index[J];海洋环境科学;2014-01
2 FENG Zhen-Zhou1,2,YU Ren-Cheng1,ZHOU Ming-Jiang1 1(Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071,China) 2(Graduate School,Chinese Academy of Sciences,Beijing 100039,China);Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum LimaDodge[J];分析化学;2012-04
3 JIANG Tao1,WANG Rui1,WU Ni1,2,JIANG Tian-jiu1,2(1.Research Center of Harmful Algae & Aquatic Environment,Jinan Unversity,Guangzhou 510632,China;2.College of Life Science,South China Normal University,Guangzhou 510630,China);Study on Hemolytic Activity of Chattonella marina Hong Kong Strain[J];环境科学;2011-10
4 CHEN Yang , YAN Tian **, YU Rencheng , ZHOU Mingjiang Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Toxic effects of Karenia mikimotoi extracts on mammalian cells[J];Chinese Journal of Oceanology and Limnology;2011-04
5 Hongxia Wang,Douding Lu*,Haiyan Huang,Xinfeng Dai,Ping Xia Laboratory of Marine Ecosystem and Biogeochemistry,Second Institute of Oceanography,State Oceanic Administration,Hangzhou 310012,China;Morphological and Phylogenetic Analysis of Karlodinium veneficum Isolated from the East China Sea in China[J];植物学报;2011-02
6 AN Xin-long et al (Laboratory of Harmful Algae and Aquatic Environment,Ocean College of Hebei Agricultural University,Qinhuangdao,Hebei 066003);Review on Chattonella marina[J];安徽农业科学;2010-32
7 YAN Xin-ya,YANG Wei-dong,TAN Shao-zao,LI Hong-ye, YU De-ren,LIU Jie-sheng (College of Life Science and Technology,Jinan University, Guangzhou 510632,China);On the effects of different organic modified clays on removing Chattonella marina[J];安全与环境学报;2010-03
8 LIU Wang-peng,HAUNG Juan,YANG Wei-dong,LI Hong-ye,CUI Wei-min,LIU Jie-sheng College of Life Sciences and Technology,Jinan University,Guangzhou 510632;Effect of Some Nutrients on the Growth and Haemolytic Toxins Production of Chattonella marina[J];生态毒理学报;2010-03
9 WEI Qin-sheng1,2,ZHAN Run1,2,ZANG Jia-ye1,2,LI Rui-xiang1,2(1.Research Center for Marine Ecology,First Institute of Oceanography,State Oceanic Aaminitration Peope’s Republic of Chnia,Qingdao 266061,China;2.Key Lab of Science and Engineering for Marine Ecological Environment,State Oceanic Aaminitration Peope’s Republic of Chnia,Qingdao 266061,China);Distributions and influence factors of the chemical parameters in the Southern Yellow Sea in spring[J];海洋科学;2010-03
10 PENG Yinghui,LIU Jiesheng,LI Hongye,HUANG Zhanchao,LI Xiuqin,YANG Weidong School of Life Science and Technology,Jinan University,Guangzhou 510632,China;Studies on performance standard for hemolytic toxins in harmful bloom algae[J];卫生研究;2009-06
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 ;The research trend of Karenia mikimotoi[J];海洋科学;2015-09
2 CHEN Bao-hong;XIE Er-yi;GAO Ya-hui;JI Wei-dong;ZHOU Qiu-lin;Third Institute of Oceanography,State Oceanic Administration;Third Institute of Oceanography,State Oceanic Administration,Xiamen 361005,China;School of Life Science of Xiamen University;;Toxic effects of red tide caused by Karenia mikimotoi on marine organisms[J];福建水产;2015-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Zhao-Hui,QI Yu-Zao,CHEN Ju-Fang and XU Ning(Institute of Hydrobiology,Jinan University,Guangzhou 510632);POPULATION DYNAMICS OF CHATTONELLA IN SPRING IN DAYA BAY,THE SOUTH CHINA SEA AND THE CAUSE OF ITS BLOOMS[J];水生生物学报;2006-04
2 LIU Jie-Sheng~1,PENG Xi-Chun~2,YANG Wei-Dong~1 (1.Department of Biotechnology,Ji′nan University,Guangzhou 510632,China;2. Department of Food Engineering,Ji′nan University,Guangzhou 510640,China)).;Growth and hemolytic activities of Phaeocystis globosa Scherffel at different mutrients condition[J];生态学报;2006-03
3 JIANG Hanming ZHAI Jing1 ZHANG Yuanying1 GU Hongyan1 GAO Kunshan2 1.Staff of Biochemistry, Department of Basical Medicine, Taishan Medical College, Tai'an, 271000 2. Marine Biology Institute, Science Center, Shantou University, Shantou, 515063;INFLUENCES OF TEMPERATURE ON THE GROWTH AND FATTY ACID COMPOSITION IN MARINE MICROALGAE[J];食品研究与开发;2005-06
4 SUN Jun~1, NING Xiu-ren~2 (1.Key Laboratory of Marine Ecology & Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China);MARINE PHYTOPLANKTON SPECIFIC GROWTH RATE[J];地球科学进展;2005-09
5 LI Tao, LIU Sheng, HUANG Liang-min, XU Zhan-zhou, LI Kai-zhi, SU Qiang, SONG Xing-yu, ZHANG Jian-lin(South China Sea Institute of Oceanology, The Chinese Academy of Sciences, Guangzhou 510301, China);STUDIES ON PHYTOPLANKTON COMMUNITY CHANGE AT DAYA BAY DURING A RED TIDE[J];热带海洋学报;2005-03
6 PENG Xi-chun1, YANG Wei-dong2, LIU Jie-sheng2*,PENG Zhi-ying1, DENG Rui-xia2, JIANG Tian-jiu2 (1. Food and Biological Engineering College, South China University of Technology, Guangzhou 510640, China; 2. College of Life Sciences and Technology, Jinan University, Guangzhou 510632, China);Extraction of Haemolytic Substances from Alga Phaeocystis globosa[J];热带亚热带植物学报;2005-01
7 XU Ning, L Song-hui, CHEN Ju-fang, HE Li-shi, XIE Long-chu, QI Yu-zao (Institute of Hydrobiology, Jinan University, Guangzhou510632, China);The influence of water temperature and salinity on the growth of Scrippsiella trochoidea[J];海洋环境科学;2004-03
8 Tan Yi Wang Guixue Δ Cai shaoxi (Key Laboratory for Biomechanics & Tissue Engineering of the State Ministry of Education, College of Bioengineering Chongqing University, Chongqing 400044,China);Application of Atomic Force Microscopy in Cell Biology[J];生物医学工程学杂志;2004-01
9 HUANG Xiao ping, HUANG Liang min, TAN Ye hui, ZHU Liang sheng (South Chinese Sea Institute of Oceanology, The Chinese Academy of Sciences, Guangzhou 510301, China);Relationship between red tide and environmental conditions in coastal waters[J];海洋环境科学;2002-04
10 CHEN Yong, LIU Jie-sheng, LUO Ying, et al. College of Life Science and Technol-ogy, Ji'nan University, Guangzhou 510632, China;Preliminary Research on the Methods of Fragmentation of the Cell Wall and the Toxicity of Alexandrium tamarense in Different Growth Phases[J];环境与健康杂志;2002-03
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