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《Journal of Hygiene Research》 2010-03
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Comparison of immunosuppression induced by different doses of cyclophosphamide in normal mice

QI Lijuan,SONG Yan,WANG Wei,CUI Wenming,ZHANG Xin,LIU Zhaoping,SUN Nana,LI Ning Institute for Nutrition and Food Safty,China CDC,Beijing 100021,China  
Objective To compare the methods for developing immune-suppressed mice models induced by cyclophosphamide(CTX) with different dosages and ways.Methods The mice were assigned to three groups;distilled water were given by gavage daily to the mice in control group;60 mg /kg of CTX were given by gavage daily to the mice in CTX-1 group;and the mice in CTX-2 group were intraperitoneally injected(ip) with 200 mg /kg bw of CTX one day before the end of the experiment.The experiment lasted for 30 days.Results The body weight,spleen weight,the percentage of lymphocytes in peripheral blood,activities of natural killer cells(NK) and cytotoxic T lymphocytes(CTL) and HC50 in CTX-1 group were significantly lower than those in the control group(P 0.05).The percentage of T lymphocytes in peripheral blood,the percentage of Th and Ts lymphocytes and Th /Ts ratio in CTX-2 group were significantly higher than those in the control group(P 0.05).Other immunological indices in CTX-2 group were significantly lower than the control group(P 0.05).Conclusion A proper immunosuppressed animal model for the functional food evaluating system could be established by gavaging 60 mg /kg bw of CTX for 30 days or by one intraperitoneal injection(ip) of 200 mg /kg.But the former might be more fitted to establish the immunosuppressed model.
【Fund】: “十一五”国家科技支撑计划项目(No.2006BAK02A07)
【CateGory Index】: R-332
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