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《Acta Academiae Medicinae CPAF》 2011-12
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Experimental study on the source bacteria in sterile packages for war readiness

ZHANG Ying,WANG Xian-yuan,ZHOU Juan(Beijing Municipal Corps Hospital of Chinese People's Armed Police Force,Beijing 100027,China)  
【Objective】 To investigate the source of bacteria in sterile packages for war readiness under natural environment in highland region.【Methods】 Genomic DNAs of Staphylococcus aureus,Staphylococcus xylose,Bacillus cereus and Staphylococcus hominis from sterile packages for war readiness and air bacteria strains in war readiness warehouse were extracted,and then used as templates to amplify the 16SrRNA and 23SrRNA internal transcribed spacer(ITS) sequences by PCR.PCR products were extracted and sequenced,and the sequences homology analysis was performed with BLAST.【Results】 Amplification products of DNA PCR from Staphylococcus aureus,Staphylococcus xylose,Bacillus cereus and Staphylococcus hominis were about 1000 bp,1100 bp,850 bp and1000 bp respectively;The 16SrRNA-23SrRNA ITS sequences of bacteria strains in sterile packages shared 99 % to 100 % homology with those from the air bacteria strains in war readiness warehouse.【Conclusion】 The bacteria in sterile packages for war readiness come from the air in war readiness warehouse under natural environment.
【CateGory Index】: R826.2
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