Effect of High Osmolarity on Electrotransformation Efficiency of Bacteria
XU Min,MA Jun-shuang,WANG Zheng-xiang(School of Biotechnology, Southern Yangtze University, Wuxi 214036, China)
The effect of hyperosmolarity, such as sorbitol,mannitol,glycerol,sucrose and glucose on electrotransformation of the gram-negative Escherichia coli JM109 and the gram-positive Bacillus licheniformis 0204 were studied. Recovery media containing 0.7 mol/L mannitol protected the cells of Bacillus licheniformis well, and so did 0.1 mol/L sucrose for E. coli JM109. At the optimal electrotransformation condition, the highest transformation efficiency of 9.8×10~(2) transformants/μg DNA was obtained for Bacillus licheniformis, and 5.9×10~(8 )transformants/μg DNA for (E.coli) JM109.
【CateGory Index】：
Q933
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