Effects of D-Biotin and VB_1 Factors on the Biosynthesis of Valine in a Valine Producer Corynebacterium glutamicum AATV341
YANG Yi, ZHANG Zhi-lan, JIANG Xu-lin, DU Da-qing, ZHANG Xing-yuan(The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036,China)
In this paper, the effects of D-biotin and VB_(1) on overproduction of valine were studied, and the composition of culture medium was also optimized. The concentrations of several metabolites produced in batch cultire (extra-cellular and intra-cellular) were determined. The distributions of carbon-flux, especially at the node pyruvate were calculated, compared, and analyzed. The results showed that,in Corynebacterium glutamicum AATV341 cultivation, adding 40 (μg/L) biotin and 400 μg/L VB_(1) would increase the productivity of valine, and repress the extra-cellular concentrations of oxaloacetic acid, acetic acid and glutamate. The analysis may play an important role in re-designing metabolism to achieve further fermentative improvement.
【CateGory Index】： Q936