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《Journal of Food Science and Biotechnology》 2008-02
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Isolation and Purification of Polyphenol Oxidase from Fresh-Cut Lotus Root

PAN Yong-gui1,2, CHEN Wei-xin2(1.College of Food,Hainan University,Hainan 571737,China;2.College of Horticulture,South China Agricultural University,Guangdong Province Key Laboratory of Postharvest Physiology and Technology of Fruits and Vegetables,Guangzhou 510642,China)  
Enzyme browning is one of the most reactions impacting quality of fresh-cut lotus root.Polyphenol oxidase is a key enzyme induing enzyme browning.Polyphenol Oxidase in fresh-cut lotus root was extracted by phosphate buffer and purified by ammonium sulfate precipitation,ion exchange chromatography using DEAE Sepharose column,and Phenyl-Sepharose 6 Fast Flow hydrophobic chromatography.PPO from fresh-cut lotus root was purified by 95.66 –fold and a 2.4% yield.The enzyme was determined to be homogenous by SDS-PAGE.The molecular weight of the fresh-cut lotus root PPO was estimated by SDS-PAGE ranged from 65 900 to 66 100 according to Sephadex G-100 gel fractionation.
【Fund】: 华南热带农业大学科技基金项目(Rnd0618);; 海南省热带园艺重点实验室开放课题资助项目(ch002)
【CateGory Index】: TS255
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【References】
Chinese Journal Full-text Database 2 Hits
1 GAO Han1,SUN Jun-liang1,GAO Yuan-jun2,NAN Hai-juan1,LIU Qian1 (1.College of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,China; 2.School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China);Effect of Modified Atmosphere Packaging on Preservation of Fresh-cut Lotus Roots[J];Food Science;2008-10
2 LIANG Jian-rong,HUANG Jie,SU Mo,GAO Ya-peng,TANG Yun-ming1.School of Life Science,Southwest University,Chongqing 400715,China;2.Chongqing Sweetpotato Research Center,Chongqing 400715,China;3.Key Laboratory of Eco-environments in Three Gorges Reservoir Region Ministry of Education,Chongqing 400715,China;Isolation,Purification and Enzymological Characterization of Polyphenol Oxidase from Sweet Potato Leaves[J];Journal of Southwest University(Natural Science Edition);2011-02
【Citations】
Chinese Journal Full-text Database 8 Hits
1 Wang Sanhu; et al.(Food Processing Department);Studies on polyphenol Oxidase Main Chracters in Lotus Root and Prevention of Discoloration[J];JOURNAL OF HENAN VOCATION-TECHNICAL TEACHERS COLLEGE;1994-01
2 LI Ning, YU Zhi fang, ZHAO You xing, XIA Zhi hua, PENG Gui xia (College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China);Enzymological Property of Polyphenoloxidase(PPO) in Lotus Root[J];Jiangsu Journal of Agricultural Sciences;2002-01
3 Yang Ming;Wang Zhijun(Dept.of Food,Sci,Jiansu Agric,Coll.,Yangzhou 225009);STUDY ON CHARACTERS OF POLYPHENOL OXIDASE IN LOTUS ROOT[J];;1996-01
4 Hu Jun(Department of Food Science Zhejiang Agriculture University, Hangzhou);The Characters of Polyphenol Oxidase in Lotus Root and Prevention of Discoloration of Lotus Root[J];Food and Fermentation Industries;1989-03
5 MENG Guang-rong,YANG Shu-lin~(*),ZENG Liang-liang,LI Xin-zhu, CAI Feng(Biotechnology Institute,Nanjing University of Science and Technology,Nanjing 210094,China);Purification and Properties of an Endo-β-glucanase[J];Journal of Food Science and Biotechnology;2006-02
6 Alex Koku M;Yang Fangqi;Gao Fuchen(Dept.of Food Science and Eng. );Recherche on Guangdong Zhima Banana Browning(Ⅰ)---Study on the Extraction,Purification and Characteristics of the PPO[J];Journal of Wuxi University of Light Industry;1994-01
7 DUAN Yu-quan1, FENG Shuang-qing2, MA Qiu-juan3, ZHAO Yu-mei2(1Science Academy of State Administration of Grain, Beijing 100037 ; 2College of Food Science and Nutritional Engineering,China Agricultural University, Beijing 100094; 3State Administration of Knowledge Property Right, Beijing 100083);Purification of Polyphenol Oxidase of ‘Zhonghuashoutao Peach Flesh[J];Scientia Agricultura Sinica;2004-04
8 Lin Zhi-fang Li Shuang-shun Zhang Dong-lin Liu Shu-xian Li Yue-biao Lin Gui-zhu Chen Mian-da (South China Institute of Botany,Academia Sinica Guangzhou);THE CHANGES OF OXIDATION AND PEROXIDATION IN POSTHARVEST LITCHI FRUIT[J];植物学报(英文版);1988-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LUO Kai-ming(Heyuan Polytechnic of Guangdong Province,Heyuan 517000,China);Gene Clone of Polyphenol Oxidases in Tea[J];Shandong Agricultural Sciences;2009-08
2 CAO Jun-sheng et al (Life Science College,Yanan University,Yanan,Shanxi 716000);Effect of CaCl_2 and 6-BA on the Changes of Internal Subtances in Chinese-date Variety[J];Journal of Anhui Agricultural Sciences;2005-02
3 ZONG Mei et al ( College of Life Sciences, Anhui Agriculture University, Hefei, Anhui 230036);Effective Factors of Browningand Relationship between Browningand Desiccation Toleranceduring the Development of Chestnut Seed[J];Journal of Anhui Agricultural Sciences;2005-08
4 OU Yan et al (College of Horticulture,Northwest Sci-tech University of Agriculture and Forestry,Yangling,Shaanxi 712100);Effect of Heat Shock Treatment on Fresh-keeping of Mini-processing Broad Bean Seed[J];Journal of Anhui Agricultural Sciences;2006-05
5 PAN Yong-gui et al(Department of Food Science and Engineering,South-China University of Tropical Agriculture,Danzhou,Hainan 571737);Study on the Enzyme Activity Correlated to Browning in the Fresh-cut Chinese Water-chestnut[J];Journal of Anhui Agricultural Sciences;2007-31
6 FAN Min et al(College of Life Sciences and Engineering,Southwest Jiaoda University,Chengdu,Sichuan 610031);Research Progress on Fructus Canarii[J];Journal of Anhui Agricultural Sciences;2010-34
7 LIU Chun-li et al(Sichuan University of Science & Engineering,Zigong,Sichuan 643033);Study on Enzymatic Characteristics of Polyphenol Oxidase(PPO) in Litchi Pulp[J];Journal of Anhui Agricultural Sciences;2011-02
8 XU Jin-yun1,WANG Qing-zhang 1,2,HE Jian-jun2,ZHOU Ming-quan2,HU Zhong-li 2 (1.College of Food Science,Huazhong Agricultural University,Wuhan 430070; 2.Lotus Research Centre,Wuhan University,Wuhan 430072, China);Study on Storage and Physiology of Enlarged Rhizome of Lotus[J];Amino Acids & Biotic Resources;2003-02
9 XU Xiao-chun,LIN Chao-peng,XU Qi-zuan(Yingdong College of Bio-engineering,Shaoguan College,Shaoguan,Guangdong 512005,China);Effects of Several Antistaling Agents Treatment on Storage of Fresh-cut Lotus Roots[J];Northern Horticulture;2008-02
10 MU Cui-e1,RAO Jing-ping2,ZHANG Zhong-hai 3(1.Shanxi Agriculture Infermation College,Taigu,Shanxi 030801;2.College of Horticulture,Northwest Agricultural and Forestry University,Yangling,Shaanxi 712100;3.Jiaozuo Teachers College,Jiaozuo,Henan 454002);Effect of Storage Treatment with N_2 on Shelf-life of the Fresh-cut Kiwifruit Slice[J];Northern Horticulture;2010-18
China Proceedings of conference Full-text Database 6 Hits
1 Guan Junfeng(Hebei Agrotechni- cal Teachers College,Changli, Hebei 066600);The relationship between core browning and peroxidation in 'Yali' pears[A];[C];1994
2 Zhang Zhaoqi, and Pang Xuequn (Department of Horticulture,Insititute of Biotechnology, South-China Agricultural University, Guangzhou 510642);The Mechanism of Litchi Pericarp Browning[A];[C];1998
3 Lan Bifeng and Zhang Jinyun ( Food Sci. Dept., Zhongkai Agrotechnical College, Guangzhou 510225);Progress in the Research on Post-harvest Physiology and Storage Techniques of Nelumbo nucifera Gaertn[A];[C];2002
4 Qiu Dongliang and Pan Dongming ( College of Horticulture , Fujian Agriculture and Forestry University Fuzhou 350002);The Advance in Postharvest Physiology and Storage Techniques of Chinese Olive[A];[C];2002
5 ;节瓜种子发芽过程中乙烯释放、保护性酶活性与植株花性别分化的相关分析[A];[C];2006
6 Jiang Hongbo~(1,2) Wang Zejin~(1,2) Lin Qixun~(1,2) 1.College of Food Science,Fujian Agriculture andForestry University,Fuzhou 350002 2.Fujian Research Center of Agricultural Product Processing,Fuzhou 350002;The treatment with O_3 in different Length of time have influence on the cold Storage quality of Agrocybe aegerita[A];[C];2006
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhang Li Chen Naifu(Department of Chemistry and Life Science,West Anhui University,Liuan 237012,china);Study on Enzymatic Characteristic of Polyphenol Oxidase(PPO) and its Application in Control of Enzymatic Browing[J];Anhui Agricultural Science Bulletin;2006-12
2 WU De-chuan et al (Agriculture College,Anhui Agricultural University,Hefei,Anhui 230026);Researeh Advances in Polyphenol Oxidase in Tobacco Plants and the Relations between PPO and Tobacco Leaf Quality[J];Journal of Anhui Agricultural Sciences;2006-07
3 YANG Chao-zhu,MA Chuan-xi,HUA Wei,XU Chuan-mei (Department of Agronomy,Anhui Agricultural University,Hefei 230036);The Distribution of PPO in Wheat Plant[J];Journal of Anhui Agricultural University;2002-04
4 YAN Shou -lei,WANG Qing -zhang,PENG Guang -hua (Food Science Department,Huazhong Agricultural University,Wuhan 430073,China);Exploration of Boiled Lotus Root Slices Processing Technology[J];Storage and Process;2002-03
5 GAO Hai-yan1, ZENG Jie1, ZHU Ji-ying2(1.Liaoning Agricultural Vocation-technic College, Xiongyue 115214 , China; 2.Shandong University of Technology, Zibo 255012, China);Study on Being Kept Green and Brittle of Packed-Lettuce[J];Storage and Process;2004-04
6 Zhao Donghai, Wang Yun, Zhang Jianping;Studies on the Biochemical Conditions of Browning Promoted by Enzyme in Fruit and Vegetable[J];Journal of Changjiang Vegetables;2005-07
7 Zhang Xueyi1,Jin Jianli2,Feng Lei1,Yu Shuang2,Zhang Jing2,An Wenhe2,Yao Hongwei1,Xie Chenyang1,Li Maohua2(1.Heilongjiang Institute of Forest By-product and Speciality,Key laboratory of Forest By-productand speciality,Heilongjiang Mudanjiang,157011;2.Mudanjiang Teachers college);Aralia elata Acquisition Process in the PPO Activity of Research[J];Forest By-Product and Speciality in China;2008-05
8 WANG Xue-jing,ZHANG Zi-de,CHEN Zhi-zhou,LI Xiang-li (College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China);Study on the factors influencing the polyphenoloxidase activity in peach fruit[J];Journal of Agricultural University of Hebei;2005-03
9 ZHAO Dong, LIU Zu_sheng, XI Biao (Department of Tea Science, Zhejiang University, Hangzhou 310029,China);Cloning and Alignment of Polyphenol Oxidase cDNA of Tea Plant[J];Journal of Tea Science;2001-02
10 Xie Shaoping1 Ouyang Xuezhi2 (1. Zhongshan College, UEST of China Guangdong Zhongshan 528403; 2. Key Laboratory of Gene Engineering of The Ministry of Education, Zhongshan University Guangzhou 510275);A Study on Control of Enzymatic Browning in Banana Processing[J];Journal of University of Electronic Science and Technology of China;2003-06
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 Wang Xiangyang, Jiang Lijia, Wang Zhongying (College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, China);Enzymatic browning of lotus roots and its control during storage[J];Transactions of the Chinese Society of Agricultural Engineering;2009-04
2 Zhang Yuejin* Hao Xiaoyan* Liang Zongsuo Lin Wenchao Guo Hongbo** College of Life Sciences,Northwest Agriculture and Forestry University,Yangling 712100;Cloning of Polyphenol Oxidase Gene(PPO) from Lotus Rhizome Bud and Its Expression in Different Tissues[J];Journal of Agricultural Biotechnology;2011-04
3 ZHU Mei-yun DANG Jian-lei WEI Zheng LIN Shun-shun(College of Food Science and Technology,Henan Agricultural University,Zhengzhou,Henan 450002,China);Research of chitosan compound film on fresh-cutting lotus root[J];Food & Machinery;2010-01
4 Sun Xiaofei,Ding Yongchuan,Ma Jianping(Zaozhuang Vocational College,Zaozhuang 277800,Shandong,China);Effects of Chitosan on Fresh Cut Lotus Root Browning and Antioxidant Activities[J];Crops;2011-02
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 ZHANG Zhi-qiang 1, GUO Chun-teng 1, LIN Jia-ren 2, RAO Ping-fan 1 (1. Institute of Biotechnology, Fuzhou University, Fuzhou,Fujian 350002, China; 2. Guizhou Laolaifu Pharmacy, Guiyang, Guizhou 550025, China);Isolation and purification of an endoglucanase from Aspergillus niger[J];Journal of Fuzhou University(Natural Sciences Edtion);2002-05
2 Feng Xin;Zhou Hongwei(Food Science Department,Shandong Agricultural University, Taian,China 271018);PARTIAL PURIFICATION AND CHARACTERISTICS OF POLYPHENOL OXIDASE FROM PRUNUS PERSICA L.(FEICHENG PEACH)[J];JOURNAL OF SHANDONG AGRICULTURAL UNIVERSITY;1996-01
3 Hu Jun(Department of Food Science Zhejiang Agriculture University, Hangzhou);The Characters of Polyphenol Oxidase in Lotus Root and Prevention of Discoloration of Lotus Root[J];Food and Fermentation Industries;1989-03
4 Chen Jianiun et al;Study on the Enzyme Browning Mechanism of Pingguo Pear and Duck Pear[J];FOOD SCIENCE;2000-02
5 SHI Xiang zhu, GUO Chun teng, ZHOU Jian wu, WANG Zhong lai, RAO Ping fan (Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China);Separation and Purification of Cellulase Using Affinity Membrane[J];Chinese Journal of Chromatography;2002-04
6 Tan Xing-jie;Zhou Yong-chen South China Institute of Botany, Academia Sinica;STUDIES ON THE ENZYMATIC BROWNING OF Litchi chinensis PERICARP BY POLYPHENOL OXIDASE[J];;1987-02
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