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Ginkgo biloba extract (Ginaton) attenuates acute lung injury induced by lipopolysaccharide in mice

GUO Zhengming1,CHEN Yi2,LIU Huijun2,XIAO Yuequn1,SONG Haipeng1,WANG Zhaozhao1,QU Xiaolin1,LUO Ziqiang2(1.Department of Basic Science,Hunan Traditional Chinese Medicine College,Zhuzhou Hunan 412012;2.Department of Physiology,Xiangya School of Medicine,Central South University,Changsha 410078,China)  
Objective To determine the protective effect of Ginkgo biloba extract (Ginaton) on acute lung injury induced by lipopolysaccharide (LPS) and its protective mechnism in mice.Methods The Kunming mice were randomly allocated into sham,LPS,Ginaton,and Ginaton+LPS groups.LPS was intraperitoneally injected at a dose of 10 mg/kg body weight in the LPS group,along with equal volumes of saline in the sham group.Ginaton+LPS group and Ginaton group were treated with Ginaton (100 mg/kg,intraperitoneally) 2 h prior to the injection of LPS or saline,respectively.The mice were killed 8 h after LPS challenge The bronchoalveolar lavage fluid (BALF) samples were analyzed for the concentration of total protein,lactate dehydrogenase (LDH) activity.The lung samples were taken for histologic evaluation and for determination of wet-to-dry (W/D) lung weight,malondialdehyde (MDA) level,myeloperoxidase (MPO) activity,nitric oxide (NO) level,and inducible nitric oxide synthase (iNOS) activity,as well as immunohistochemistry analysis for iNOS and heme oxygenase-1 (HO-1) protein expression.Results All the indexes except HO-1 in the ginaton group were not significantly different to those in the control group.But lung tissue and BALF showed serious inflammatory changes in the LPS group.Compared with the LPS group,in addition to inflammatory reaction,the changes of protein content and LDH in BALF and W/D,level of MDA and NO,activity of MPO and iNOS in lung tissues were significantly reduced in the Ginaton+LPS group.(P0.05 or P0.01).The expression ofiNOS protein in lung tissues was obvious higher in the LPS group than that of the Ginaton+LPS group (P0.01);however expression of HO-1 in the Ginoton + LPS group was significantly higher than that of the Ginoton group.Conclusion Ginaton can play a protective role against LPS-induced acute lung injury and it may be associated with the up-regulation of HO-1 and the down-regulation ofiNOS expression and activity.
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