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《Journal of Wuhan Botanical Research》 2004-03
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Study on Transformation of Insecticidal Bacillus thuringiensis cryIAc Gene in Pepper

YUAN Jing1,SHU Qing-Yao2,LIU Zhong-Lai1(1.Molecular Biology Laboratory,Central China Normal University,Wuhan 430079,China;2.Institute of Nuclear Agricultural Sciences,Zhejiang University,Hangzhou 310029,China)  
The insecticidal Bacillus thuringiensis (Bt) cryIAc gene was used to transform the cotyledon explants of pepper via Agrobacterium transformation.Through stringent selection of kanamycin,analysis of the expression of GUS in the plants by histochemical method,molecular biological assays with PCR and Southern dot hybridization,it is confirmed that the cryIAc gene was integrated into the genome of pepper.
【CateGory Index】: Q943
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