Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Wuhan Botanical Research》 2006-04
Add to Favorite Get Latest Update

Polyclonal Antibody Preparation and Expression Analysis of High-affinity K~+ Transporter SsHKT1

SHAO Qun~1,HAN Ning~(1,2),DING Tong-Lou~1,WANG BaoShan~1~~*(1.College of Life Science,Shandong Normal University,Ji'nan 250014,China;2.College of Food and Biologic Engineering,Shandong Institute of Light Industry,Ji'nan 250100,China)  
We isolated a cDNA homologous to HKT1 from Suaeda salsa L.(SsHKT1) roots using RT-PCR and RACE methods.The hydrophilic fragment(403-612 bp) was amplified by PCR from SsHKT1 cDNA of halophyte S.salsa L.PCR product was inserted into prokaryotic expression vector pGEX-6P-3.The recombinant vector was transformed into Escherichia coli BL21(DE3) and the GST-SsHKT1 fusion protein expression was induced by IPTG.The fusion protein was mainly in inclusion bodies.The purified protein was injected into a New Zealand rabbit and the rabbit's anti-serum against SsHKT1 with higher specificity was prepared.Western-blot analysis using this polyclonal antibody demonstrated that SsHKT1 may be localized to the plasma membrane.Further study shows that level of SsHKT1 proteins increase under conditions of K~+ starvation or salt stress.These datas suggest that SsHKT1 is important for K~+ uptake of S.salsa L.and particularly for K~+ nutrition under high salt conditions in this plant.
【Fund】: 国家自然科学基金资助项目(30270793);; 山东省自然科学基金重点项目(Z2004D03)资助
【CateGory Index】: Q945.78;Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved