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《Journal of Wuhan Botanical Research》 2007-02
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Cloning and Analysis of a cDNA Encoding Key Enzyme Gene (hmgr) of the MVA Pathway in Medicinal Plant Euphorbia pekinensis

CAO Xiao-Ying1,JIANG Ji-Hong1,LIU Qun1,DAI Chuan-Chao2,CHEN Feng-Mei1(1.Xuzhou Normal University,Key Laboratory of Biotechnology for Medicinal Plant,Xuzhou,Jiangsu 221116,China;2.College of Life Sciences,Nanjing Normal University,Nanjing 210097,China)  
Based on the design of degenerated oligonucleotides according to the conservative regions of eight 3-hydroxy-3-methylglutaryl-CoA reductases from six plants and total RNA extracted from Euphorbia pekinensis,a 458 bp fragment of hmgr was obtained using reverse transcription PCR strategy.Through sequence analysis by BlastP online,the resulting protein showed high homology to 3-hydroxy-3-methylgluta-ryl-CoA reductase,with 90% identification compared with Eucommia ulmoides(AAV54051),86% with Andrographis paniculata(AAP14352),86% with Picrorhiza kurrooa(ABC74565),92% with Hevea brasiliensis(AAU08214),87% with Gossypium barbadense(ABC71314)and 88% with Gentiana lutea(BAE92730).Deduced amino acid sequence were also analyzed by PROSITE,ClustalX and Phylogenic tree,and data present evidence for the existence of 3-hydroxy-3-methylglutaryl-CoA reductase in E.peki-nensis,which is the first report of the hmgr gene isolated from E.pekinensis.
【Fund】: 徐州师范大学科研重点项目(04XLA14)资助
【CateGory Index】: Q943.2
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【References】
Chinese Journal Full-text Database 2 Hits
1 CAO Xiao-Ying1 JIANG Ji-Hong1 JU Xiu-Yun1 DAI Chuan-Chao2(1.Xuzhou Normal University,Key Laboratory of Biotechnology for Medicinal Plant,Xuzhou 221116)(2.College of Life Sciences,Nanjing Normal University,Nanjing 210097);Cloning and Analysis of hmgr Gene conserved fragments in Euphorbia pekinensis Rupr[J];Bulletin of Botanical Research;2008-06
2 YANG He GAO Yu-gang LI Fan-ying ZHANG Lian-xue(Jilin Agriculture University College of Chinese Traditional Medicine,Changchun 130118,China);Research Progress on Biosynthetic Pathway of Terpenoids Containing Ginsenoside and the HMGR[J];China Biotechnology;2008-10
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Zhuang Junping1 Su Jing2 Chen Weixin1*1 Key Lab of Fruit and Vegetable Storage of Guangdong Province, Horticulture College of Sourth China Agricultural University, Guangzhou, 510642; 2 Life Science College of Zhongshan University, Guangzhou, 510275;An Effective Method for High-quality RNA Isolation from Banana Fruit[J];Molecular Plant Breeding;2006-01
2 LY Kong and ZD Min (Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009);STUDIES ON CHEMICAL CONSTITUENTS OF ROOTS OF EUPHORBIA PEKINENSIS[J];ACTA PHARMACEUTICA SINICA;1996-07
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GUO Sheng-hu et al(Key Laboratory of Agricultural Bio-technology of Ningxia Academy of Agriculture and Forestry,Ningxia,Yinchuan 750002);Study on the Chemical Constituents in the Roots of Euphorbia sieboldiana[J];Journal of Anhui Agricultural Sciences;2009-15
2 MENG Na~1, JIANG Ji-hong~2, ZHOU Shou-biao~1(1.College of Life Science, Anhui Normal University, Wuhu 241000,China;2.Key Laboratory of Medicine Plant and Biotechnology,Xuzhou 221116,China);SCREENING STUDIES ON FUNGISTASIS OF PLANT EXTRACTS OF EUPHORBIA OF ANHUI[J];Journal of Anhui Normal University(Natural Science);2005-03
3 ZHU Xiao-qin,JING Xiao-le,FENG Yong-qing,SHEN Yuan-yue*(Depertment of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China);An Effective Method for Isolation of High-quality Total RNA from Fruit Pulps[J];Journal of Beijing University of Agriculture;2008-02
4 Li Hao1,2 Zhang Pingping2,3 Zha Xiangdong1 He Zhonghu2,4 Xia Xianchun2* 1 School of Life Science Anhui University,Anhui Key Laboratory of Eco-engineering and Bio-technique,Hefei,230039;2 Institute of Crop Science,National Wheat Improvement Center,Chinese Academy of Agricultural Sciences,Beijing,100081;3 College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,430070;4 CIMMYT China Office,Beijing,100081;A Method for Rapid Isolation of High-Quality Total RNA from Wheat Seeds[J];Molecular Plant Breeding;2006-06
5 Wu Guoqiang Xi Jiejun Zhou Xiangrui Ma Qing Wang Suomin School of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730000;An Improved Method for Extracting Total RNA from Leaves and Roots of the Succulent Xerophyte Zygophyllum xanthoxylum[J];Molecular Plant Breeding;2008-01
6 CHEN Di,FU Xian-ying,YIN Xiao-min,ZHENG Rong-yi(College of Environment and Plant Protection,SCUTA,Danzhou 571737,China);Assessment of several extraction methods of total RNA from fruits[J];Guangdong Agricultural Sciences;2007-11
7 GAN Guo-ying,QI Yan-xiang,PU Jin-ji,ZHANG Xin,XIE Yi-xian(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou 571737,China);Improved CTAB method for extracting high quality total RNA from banana leaves[J];Guangdong Agricultural Sciences;2009-07
8 ZHAO Ji-rong1,BI Yang2,LIU Hong-xia3,GAO Xiong-jie2(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;2.College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;3.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Factor screening of RNA extraction from muskmelon fruit by modified Trizol method[J];Journal of Gansu Agricultural University;2009-02
9 MENG Na1, ZHOU Shou-biao 1* , JIANG Ji-hong2 ( 1.College of Life Science, Anhui Normal University, Wuhu 241000, China; 2.Key Laboratory of Medicine Plant and Biotechnology, Xuzhou 221116, China );Sequence analysis based on ITS region of nuclear ribosomal DNA and comparative anatomy on the leaves of five species in Euphorbia[J];Guihaia;2006-01
10 LIU Jing-zhi,WANG Li(Cuisine and Nutrition Education Section,Department of Tourism,Professional Skills College,Hebei Teachers University,Shijiazhuang 050031,China);STUDIES ON CHEMICAL CONSTITUENTS OF HEDYOTIS DIFFUSA WILLD[J];Journal of Hebei Medical University;2007-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Qiang et al (College of Bio & Food Technology,Dalian Institute of Light Industry,Dalian,Liaoning 116034);Research Progress in Genetic Analysis of the Biosynthesis of Phytosterols and Triterpene Saponins[J];Journal of Anhui Agricultural Sciences;2006-19
2 LAN Wen-zhi,YU Long-jiang*,CAI Yong-jun,LI Mo-yi (School of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China);Advance on molecular biology of isoprenoids non-mevalonate pathway[J];Acta Botanica Boreali-occidentalia Sinica;2001-05
3 LI Wen-quan,WANG Zi-hua,SHEN Rui-lin(Collage of Ani mal Science and Technology,Shanxi Agrcultural University,Taigu,Shanxi,030801,China);Structure and Regulation of HMG-CoA Reductase[J];Progress in Veterinary Medicine;2006-02
4 Chen Jian Zhao Degang*Guizhou key Laboratory of Agricultural Bioengineering, Guizhou University, Guiyang, 550025;Research Advances on the Enzymes and their Coding Gene Involved in Plant Terpene Biosynthesis[J];Molecular Plant Breeding;2004-06
5 LI Rong, WANG Zhe-zhi ( College of Life Sciences, Shaanxi Normal University, Xi’an 710062, China );A bioinformatics analysis on 3-hydroxy-3-methylglutaryle-CoA reductase, a key enzyme in plant isoprenoid biosynthesis[J];Guihaia;2006-05
6 ZHANG Fucheng CHEN Shoucai (Rubber Research Institute / Ministry of Agriculture Key Laboratory for Tropical Crop Physiology, CATAS, Danzhou, Hainan 571737);Progress on the Key Enzymes and Related Genes of Natural Rubber Biosynthesis Pathway from Hevea brasiliensis[J];Chinese Journal of Tropical Agriculture;2006-01
7 LUO Zhi-Yong *, LU Qiu-Heng, LIU Shui-Ping, CHEN Xiang-Hui, LUO Jian-Qing, TAN Li-Jun, HU Wei-Xin * ( Molecular Biology Research Centre, Xiangya Medical College, Central South University, Changsha 410078, China );Screening and Identification of Novel Genes Involved in Biosynthesis of Ginsenoside in Panax ginseng Plant[J];Acta Biochimica Et Biophysica Sinica;2003-06
8 HAN Jun-Li~ 1,3 ,LI Zhen-Qiu~ 2,3 ,LIU Ben-Ye~3,WANG Hong~3,LI Guo-Feng~3 and YE He-Chun~ 3 1 Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China2 College of Life Sciences, Hebei University,Baoding 071002,China 3 Key Laboratory of Photosynthesis and Environmental Molecular Physiology,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;Metabolic Engineering of Terpenoids in Plants[J];Chinese Journal of Biotechnology;2007-04
9 WANG Wei,TONG Tan Jun (Department of biochemistry and molecular biology,Beijing Medical University,Beijing 100083);The Key Enzyme of Cholesterol Synthesis Pathway:HMG CoA Reductase and Disease[J];PROGRESS IN PHYSIOLOGICAL SCIENCES;1999-01
10 JIA Ning,QIU Yan,WANG Gang (College of Life Science,Hebei Normal University,Shi Jia Zhuang 050016,China);Progress of the studies on enzymes in taxol biosynthesis[J];Joural of Biology;2002-06
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 WU Qiong ZHOU Ying-qun,SUN Chao CHEN Shi-lin(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China);Progress in Ginsenosides Biosynthesis and Prospect of the Secondary Metabolic Engineering for the Production of Ginsenosides[J];China Biotechnology;2009-10
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 HU Wei-rong 1,2, ZHU Xi-ru1, WANG Zheng-xun1, ZHANG Zhao-qi2, JI Zuo-liang2 (1.School of Biology and Chemistry Engineering,Guangzhou University,Guangzhou 510405, China; 2.College of Horticulture,South China Agricultural University,Guangzhou 510642, China);Advances of research on postharvest and storage physiology of banana fruit[J];Journal of Guangzhou University(Natural Science Edition);2003-03
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