Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Wenzhou Medical University》 2018-12
Add to Favorite Get Latest Update

Over-expression of SIRT4 in inhibiting the proliferation of gastric cancer cells through G1 cell cycle arrest

HUANG Guoyu;LIN Yao;LIN Jiahao;CHEN Xuxu;LI Junjian;ZHU Guanbao;Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University;School of the First Clinical Medical Sciences, Wenzhou Medical University;Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University;  
Objective: To investigate the effect and mechanism of SIRT4 on the proliferation and cell cycle of gastric cancer cells. Methods: We constructed SIRT4-overexpressed gastric cancer cell-lines SGC-7901 and MNK45 by lentiviral-mediated transduction. On studying cell viability and proliferation activity, we noted the effects of overexpressed SIRT4 on the proliferation and clone forming ability of gastric cancer cells in vitro. In addition, we studied the cell cycle in an attempt to gain insights into the cellular mechanism. Results: Overexpression of SIRT4 significantly inhibited the proliferation and clone forming ability of the gastric cancer cells SGC-7901 and MNK45 in vitro. Furthermore, overexpression of SIRT4 induced G1 cell cycle arrest via downregulation of p-ERK, cyclin D, and cyclin E. Conclusion: SIRT4 may play a role as a tumor suppressor gene in gastric cancer.
【Fund】: 浙江省自然科学基金资助项目(LY18H160055 LQ13H 160022);; 温州市公益性科技计划项目(Y20160404 Y20160411);; 温州市科技计划项目(Y20140712)
【CateGory Index】: R735.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved