Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Xi'an Jiaotong University》 2017-12
Add to Favorite Get Latest Update

Properties of Enzymatically Crosslinked Gelatin and Fabrication of Bio-Microchannel

CHEN Ruomeng;WANG Hong;HUANG Yajiang;LIU Yaxiong;HE Jiankang;LI Dichen;State Key Laboratory for Manufacturing Systems Engineering,Xi'an Jiaotong University;  
A new strategy is adopted to construct the hydrogel microchannel with the enzymatically-crosslinked gelatin by evaluating the different concentration gelatin hydrogel,which aims to solve the difficulty of fabricating circular microchannel by soft hydrogel.The relationship of the gelatin properties(mechanical,forming and biological)with its concentration is revealed and discussed,and the molding process of gelatin hydrogel microchannel is proposed.The structural,mechanical and endothelialization properties of gelatin hydrogel microchannel are also studied.The experimental results indicate that with the increasing gelatin concentration,the mechanical property and microstructure replication property of the composite hydrogels are gradually improved,however,superior concentration causes lower biological performance.Hence,the hydrogel with 0.1 g/mL gelatin is chosen as the optimal composition to build circular microchannel. The approach for fabricating microchannel retains the original mechanical properties of the hydrogel,although certain error exists in the forming process from characterizing morphology and the dimension of the microchannel.Besides,the microchannel gelatin facilitates attaching and spreading of human umbilical endothelial cells(HUVECs)toform a uniform endothelialized layer around the channel surface.Following the evaluation of different concentrations of enzymatically crosslinked gelatin hydrogel, the biomimetic microchannel can be expected to apply to reconstruction of substantive functional organs.
【Fund】: 国家自然科学基金资助项目(51675416);; 陕西省统筹项目(2015KTTSGY-04)
【CateGory Index】: R318.08
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved