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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2004-04
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The expression of chicken interleukin 2 in Pichia yeast

Xu Jian, Fan Yongjun, Li Long, Wan Wangjun, Li Jianrong, Yu Lian (Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China)  
Objective To express recombinant chicken interleukin 2 (IL-2) in Pichia yeast eukaryotic cell expression system for a massive preparation of chicken IL-2. Methods Spleen lymphocytes taken from Xiaoshan chicken were activated by ConA in vitro. Chicken IL-2 gene was amplified and cloned into pPIC9. The pPIC9-IL-2 was transformed into Pichia yeast by electroporation. The recombinants were induced by methanol for the expression of recombinant protein. Results The protein was examined with SDS-PAGE and Western bloting. A specific molecular weight band at about 30 ku appeared. Conclusion The recombinant protein of chicken IL-2 has been expressed in Pichia yeast.
【Key Words】： chicken IL-2 pPIC9 Pichia yeast reagent of immune enhanced
【Fund】： 浙江省重点科技项目 (No .0 1 1 1 0 2 4 65);; 浙江省自然科学基金重大项目 (No .ZA0 1 0 5)
【CateGory Index】： S831
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