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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2012-04
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Inhibitory effects of siRNA on proliferation and invasiveness of gastric cancer SGC-7901 cells by blocking NF-κB signaling pathway

WANG Hong-jun,LIAO Xin-hua,CUI Fei-bo,WEI Guang-bing(Department of General Surgery,the First Affiliated Hospital, Medical School of Xi'an Jiaotong University,Xi'an 710061,Chain)  
Objective To investigate the inhibitory effects of NF-κB p65 gene targeted small interference RNA(siRNA) on the proliferation and invasiveness of gastric cancer cell line SGC-7901.Methods Chemically synthesized small interference RNA(siRNA) directed against human NF-κB p65 was transfected into gastric carcinoma cell SGC-7901 using cationic liposome LipofectamineTM 2000 as the transfecting agent.The expression of NF-κB p65 gene was detected by RT-PCR.The DNA binding activity of NF-κB subunit p65 was detected by ELISA.Transwell invasiveness test was used to examine the changes of invasive ability of SGC-7901.The proliferation capability was determined by MTT assay.Results RT-PCR results indicated that chemically synthesized siRNA directed against human NF-κB p65 could knock down the transcription and expression of NF-κB p65 gene.The difference between the RelA siRNA group and control group was significant(P0.05).After transfection,the DNA binding activity of NF-κB p65 in RelA siRNA group was much lower than that of the control group(P0.05).The invasive and proliferative abilities of SGC-7901 decreased substantially.Conclusion NF-κB p65 siRNA effectively down-regulates the expression of NF-κB p65 gene and decreases the proliferation and invasiveness of SGC-7901cells.
【CateGory Index】: R735.2
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