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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2017-06
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Immature neurons born one week before pilocarpine-induced status epileptic exhibit aberrant integration at chronic stage after SE

YUAN Bo;ZHU Kun;HU Ming;FENG Gai-feng;YANG Peng-bo;ZHANG Jian-shui;DENG Mei-ying;LIU Jian-xin;Institute of Neurobiology,School of Basic Medical Sciences of Xi'an Jiaotong University Health Science Center;Department of Human Anatomy,Histology and Embryology,School of Basic Medical Sciences of Xi'an Jiaotong University Health Science Center;Department of Neurology,the First Affiliated Hospital of Xi'an Jiaotong University;  
Objective To determine whether the integration of immature neurons born before status epilepticus(SE)can be disrupted by an epileptogenic insult.Methods Pilocarpine was used to induce SE in mice.At week 1 before induction,BrdU or retroviral vector expressing green fluorescent protein(RV-GFP)was used to label the newly born cells in the dentate gyrus(DG).At week 8 after SE,BrdU+Map2 or BrdU+NeuN doublelabeling staining was carried out to visualize hilar basal dendrite or hilar ectopic migration.Virus-transduced GFP signals were used to identify the mossy fiber sprouting from the newly generated neurons.The number of cells with aberrant integrations was compared using unpaired Student's t-test.Results The percentage of newborn neurons with aberrant dendritic morphology was(20.8±8.4)% at week8 after SE.The percentage of BrdU+NeuN double labeled cells ectopically migrated into the hilus was(15.9±7.4)%.At week 8 after SE,the chronically epileptic mice showed many GFP+processes in the IML with the same axonal appearance and small mossy fiber bouton-like structures as those seen in the hilus.The number of newborn neurons with aberrant integrations in SE mice was significantly increased when compared with the control mice(P0.05).Conclusion These data demonstrate the existence of aberrant integrations-hilar basal dendrites,hilar ectopic migration and mossy fiber sprouting in the DGgenerated cells born 1 week before an SE insult.
【Fund】: 国家自然科学基金面上项目(No.31671249 81171232)~~
【CateGory Index】: R742.1
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