Synthesis of immunomagnetic microsphere and its separation for recombinant human IL-2
Guo Li'an; Chen Wanlu; Zhu Baoquan; Chen Daijie(Central Laboratory,the Fourth Military Medical University, Xi 'an 710032)
The synthesis of immunomagnetic ndcmepbere(IMMS) of monoclonal antibodies (mAb) to recombinant human IL-2(rhIL-2) as ho to Fe3O4-po ndcmephere (aMS) and separation for rblbZ from suspendon of in ed have ben in thes Paper. minaboc ndcmephere was Preped by physics wrapper encirclement. After reaction. the tie poWer menvelope in the griddle of agarose. And then, magnehc ndcmephere was coupled with anti to form rosphere. The coupling method of mAb with MMS were CNBr methods. The Iaps was ed to ho sus of inAsion . aam party of rffe-2 by one step reached 93%,specific activity 2. 0 x 106IU/mg protein, ratio of arhvity 80%.There are two main advanbo of IMMS in seaing protein. Face, acs method can separate protein from suspension.blond, it is very simple ed fast.
【CateGory Index】： R318.08