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《Journal of Cellular and Molecular Immunology》 2002-04
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Study on SCF secretion by bone marrow stromal cells of anemic mice stimulated with astragalus membranaceus in vitro

ZHU Xiao ling 1 , ZHU Bi de 2 1 Department of Histology and Embryology, School of Basic Medical Sciences and Forensic Medicine, Medical Center of Sichuan University, Chengdu 610041, Sichuan Province, China; 2 Chengdu University of  
Aim To study effect of Astragalus membranaceus injection (AMI) on stem cell factor (SCF) secretion by bone marrow stromal cells of anemic mice. Methods Anemic mice were prepared. 0.1 mL of AMI in final concentrations (40 mg/L or 400 mg/L)was added respectively into culture system for bone marrow stromal cells from anemic mice as experimental group1 or group2. Equal quantity of IMDM was added into the same culture system as control group. Colony forming unit fibroblast (CFU F) in culture system in each group was determined. Culture supernatant of each group was collected, dialyzed and concentrated into dry powder. The freeze dry powder was diluted respectively with 1mL of IMDM and then was added to culture system of colony forming cells with high proliferative potent (HPP CFC). SCF level of culture supernatant in each group was determined. Results Number of CFU F and SCF level in bone marrow stromal cell conditioned medium (BMSCM) of experiment groups were markedly higher than those of control group. Conclusion AMI of certain concentration may promote proliferation of CFU F and increase level of SCF secreted by bone marrow stromal cells from anemic mice.
【CateGory Index】: R285
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