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《Journal of Cellular and Molecular Immunology》 2003-02
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The study of primary culture methods for hippocampal neurons of newborn rats

WANG Ying, CHE Yu, MIAO Jian ting, LI Zhu yi, WANG Zhe jin PLA Center of Neurology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China  
AIM: To compare three different methods for neuron culture, so as to provide a culture technique with higher neuron purity and survival rate. METHODS: Neurons in hippocampal region of newborn SD rats were cultured by common culture method, cytosine arabinoside (Ara c) supplementing method, Ara c and nerve growth factor (NGF) supplementing method. Then morphology of neurons was observed under microscope. The survival rates of the neurons at different culture times were compared by inverted microscope observation and MTT colorimetry. The purity of neurons cultured by 3 methods was detected by immunocytochemical staining. RESULTS: Neurons cultured by Ara c and NGF supplementing method grew well. The purity and survival rate of neurons cultured by Ara c and NGF supplementing method was highest. CONCLUSION: Ara c and NGF supplementing method is a good method for neuron culture.
【Fund】: 军队“十五”医药卫生科研基金资助项目 (No .0 1Q1 1 2 );; 第四军医大学创新工程基金资助项目 (No .CX0 1A0 1 8)
【CateGory Index】: R329
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