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《Journal of Cellular and Molecular Immunology》 2005-02
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Expression of CD14 protein in U937 cells induced by vitamin D3

SUN Hang1, WU Chuan-xin2, GONG Jian-ping 2*, LIU Hai-zhong2, LI Xu-hong2, YOU Hai-bo2, CHEN Xian-feng2 1Institute of Viral Hepatitis, 2Department of Hepatobiliary Surgery, 2nd Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China  
AIM: To observe the 1, 25-dihydroxy vitamin D3 (Vit D3) induced CD14 expression in human U937 cell line and the reaction of the cells to LPS stimulation following the induction. METHODS: U937 cells were cocultured with 0.1 μmol/L Vit D3 for 24 hours to induce the expression of CD14 gene and the sensitiveness of U937 cells to stimulation of LPS at various concentrations and for different times were observed. RESULTS: Vit D3 stably induced U937 cells to express CD14 mRNA and CD14 protein. The sensitivity of U937 cells to LPS stimulation increased notably after Vit D3 induction. It was demonstrated that low concentration of LPS stimulated the activation of NF-κB in U937/CD14 cells, and enhanced the transcription and expression of TNF-α gene, and even release of TNF-α into the culture supernatant. CONCLUSION: Vit D3 can induce U937 cells to express CD14 gene and CD14 protein and enhance the reactivity of U937/CD14 cells to LPS stimulation.
【Fund】: 国家自然科学基金资助项目(No. 30170919;; 30471696)
【CateGory Index】: R392
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