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《Chinese Journal of Cellular and Molecular Immunology》 2008-08
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Changes of cytokines in mice immunized with recombinant Bb-EmⅡ/3-Em14-3-3 vaccine of Echinococcus multilocularis

YANG Mei,LI Wen-gui Institute of Infectious and Parasitic Diseases,the First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China  
AIM: To study the effects of recombinant Bb-EmⅡ/3-Em14-3-3 vaccine on cytokine secretion in mice challenged with protoscoleces of Echinococcus multilocularis.METHODS: BALB/c mice were immunized with rBb-EmⅡ/3-Em14-3-3 vaccine by subcutaneous injection,intramuscular injection,nasal mucosa inoculation or oral administration.After 12 weeks of immunization,all the mice were challenged with 50 protoscoleces of Echinococcus multilocularis by intraperitoneal injection.18 weeks later,the mice were sacrificed and the splenocytes were stimulated with EmAg and ConA or LPS in vitro,then IL-12,IL-10,IFN-γ and TNF-α in the culture supernatant were measured by ELISA.RESULTS: The level of IFN-γ,IL-12 and TNF-α were greatly higher than that of PBS control group(P0.05 or P0.01),and the level of IL-10 was lower.The level of IFN-γ,IL-12,TNF-α and IL-10 in each group with EmAg and ConA or LPS stimulation was greatly higher than that in the group without stimulation(P0.05 or P0.01).CONCLUSION: Th1 response is induced in mice challenged with Echinococcus multilocularis by rBb-EmⅡ/3-Em14-3-3 vaccine,and the vaccine may enhance protective response against the challenge with protoscoleces of Echinococcus multilocularis.
【Fund】: 国家自然科学基金资助项目(30500423);; 教育部重点项目资助(205131);; 重庆市卫生局科研基金资助项目(05-2-203)
【CateGory Index】: R383.3
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