Effect of icariin on intermediate and advanced activation of murine T lymphocytes in vitro

TENG Fei, ZENG Yao-ying, HUANG Xiu-yan, YANG Zhi, YAO Man-lin, SONG Bing, LI LinInstitute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China  

AIM: To study the effect of icariin (ICA) on the intermediate and advanced activation of murine T lymphocytes stimulated by concanvalin A (ConA) in vitro. METHODS: Single cell suspensions were prepared from murine lymphoid nodes under germ free condition. The drug toxicity of ICA on T lymphocytes was measured by MTT after 24 h. Before they were stimulated by ConA (the final concentration was 5 mg/L), T cells were treated with different concentrations of icariin for 4 h. The expression of CD25 and CD71, the marker of intermediate and advanced activation of T lymphocytes, were measured by flow cytometry (FCM) combined with two-colour immunofluorescent staining of cell surface antigen after 24 h and 30 h. The change of cytokine contents such as IL-2, IL-4, IL-10, TNF-α and IFN-γ in the supernatant of intermediate activation was measured by LuminexTM100. RESULTS: ICA at the final concentration of 0.3, 1.5, 3.0 μmol/L inhibited the expression of CD25 with dose-dependent effect but it had no effect on that of CD71. ICA decreased the secretion of IL-2, IL-4 and IL-10 but increased that of TNF-α and IFN-γ. CONCLUSION: ICA can not only decrease CD25, IL-2, IL-4 and IL-10 but also enhance the cell-mediated immunity reaction. So it may have a function of two-way regulation of immune balance.

【Key Words】： icariin T lymphocyte CD25 CD71 FCM
【Fund】： 国家重点基础研究发展计划(973)资助项目(2006CB504201;2004CB720100);; 广东省基金项目(2006B36030016);; 广州市科技局科技攻关重点项目(2006Z-E0091);; 生物化学与分子生物学广东省重点学科资助(2008年度)
【CateGory Index】： R285.5

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