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《Medical Journal of National Defending Forces In Northwest China》 2005-05
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Molecular clone of Decorin cDNA and its expression in E.coli DH5α

YANG Guo-rong,ZHU Ren-zhi,HE Xiao-xia,et al.(Department of Pathology,The First Hospital of PLA,730030,China)  
Objective: To get human Decorin cDNA and its expression in E.coli DH5α.Methods: Decorin cDNA was got from human embryonic lung fibroblasts cell strain(2BS) cultured in vitro by nest-RT-PCR and cloned into pMD18-T vector.After sequence analysis,the mature protein region of Decorin cDNA was sub-cloned into pGEX-4T-1 vector and the fusion clone was identified by PCR,restriction enzyme incision and sequence(analysis).Then,the identified fusion clone was expressed in E.coli DH5α.Results: Decorin cDNA fragment of(1 181) bp got by us had about 99.75% similarity with Decorin cDNA sequence in Gene bank.The 1 005 bp target fragment could be amplified and got by restriction enzyme incision from the fusion clone of GST-mature Decorin cDNA.There existed a complete open reading frame in this fusion clone.The fusion protein was expressed successfully in E.coli DH5α.Conclusion: Decorin cDNA is got and the fusion protein of GST-mature Decorin is(expressed)successfully in E.coli DH5α.
【Fund】: 甘肃省科技攻关项目(GK972-2-81A);; 甘肃省自然科学基金资助项目(ZS001-A23-058-Y)
【CateGory Index】: R346
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【References】
Chinese Journal Full-text Database 3 Hits
1 YANG Guo-rong,ZHU Ren-zhi,HE Xiao-xia,et al.(Department of Pathology,The First Hospital of PLA,Lanzhou 730030,China);Preparation of decorin cDNA probe and initial detection of decorin mRNA in tissues[J];Medical Journal of National Defending Forces in Northwest China;2006-05
2 YANG Guo-rong1, ZHU Ren-zhi2, HE Xiao-xia3, XUE Yong-jie1, HE Xue-jiao1, YU Rong1(1 Department of Pathology, The First Hospital of PLA, Lanzhou 730030, China; 2 The Institute of Pathology, Lanzhou University, 3 Department of Neurosurgery, People's Hospital of Gansu Province, Lanzhou 730000, China. );Expression features of human decorin in E.coli DH5α[J];Chinese Journal of Pathophysiology;2007-06
3 YANG Guo-rong, XUE Yong-jie, JIA Xing-min, HE Xiao-xia, HE Xue-jiao, YU Rong Department of Pathology, The First Hospital of PLA, Lanzhou 730030, China;Role of decorin in liver fibrosis[J];Chinese Hepatology;2007-06
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 ;中医药对TGF-β_1调控作用的研究进展[J];Gansu Medical Journal;2008-02
2 Hepatic Institute of Shanghai University of Traditional Chinese MedicineSONG Ya-fangHONG Jia-heShuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineXU Lie-ming;Progress of the Regulation of TGF-β1 by Chinese Medicine[J];Shanghai Journal of Traditional Chinese Medicine;2004-01
3 HU Xiao-bo,CHEGN De-yun,MU Mao,NIE Li,FANG Xun,YANG Li-teng(West China Hospital,Sichuan University,Chengdu,610041,China);Study on the Protective Effect and Mechanism of Integripetal Rhodiola Herb on Rats with Pulmonary Fibrosis[J];Lishizhen Medicine and Materia Medica Research;2007-11
4 YANG Guo-rong,ZHU Ren-zhi,HE Xiao-xia,et al.(Department of Pathology,The First Hospital of PLA,Lanzhou 730030,China);Preparation of decorin cDNA probe and initial detection of decorin mRNA in tissues[J];Medical Journal of National Defending Forces in Northwest China;2006-05
5 YANG Guo-rong1, ZHU Ren-zhi2, HE Xiao-xia3, XUE Yong-jie1, HE Xue-jiao1, YU Rong1(1 Department of Pathology, The First Hospital of PLA, Lanzhou 730030, China; 2 The Institute of Pathology, Lanzhou University, 3 Department of Neurosurgery, People's Hospital of Gansu Province, Lanzhou 730000, China. );Expression features of human decorin in E.coli DH5α[J];Chinese Journal of Pathophysiology;2007-06
6 YANG Guo-rong, XUE Yong-jie, JIA Xing-min, HE Xiao-xia, HE Xue-jiao, YU Rong Department of Pathology, The First Hospital of PLA, Lanzhou 730030, China;Role of decorin in liver fibrosis[J];Chinese Hepatology;2007-06
【Co-references】
Chinese Journal Full-text Database 4 Hits
1 Sun Shaohua\ \ Zhu Renzhi\ \ Yang Guorong (Lanzhou Medical College,Lanzhou\ 730000 China);THE EFFECT OF TGF β 1 AND bFGF IN THE COLLAGEN DEPOSITION OF ALVEOLAR SEPTUM[J];JOURNAL OF GANSU SCIENCES;1999-02
2 Sun Shaohua, Yang Guorong, Sun Xiaowei et al (Dept of Pathol, Lanzhou Medical College, Lanzhou 730000);The role of pulmonary macrophages in fibrotic interstitial lung disease of human[J];CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOG;1999-05
3 YANG Guo-rong,ZHU Ren-zhi,HE Xiao-xia,et al.(Department of Pathology,The First Hospital of PLA,Lanzhou 730030,China);Preparation of decorin cDNA probe and initial detection of decorin mRNA in tissues[J];Medical Journal of National Defending Forces in Northwest China;2006-05
4 SHI Wei~1, HE Chao-sheng~1, LIU Shuang-xin~1, LIANG Xin-ling~1, YE Zhi-ming~1, LIANG Yong-zheng~2, HU Xiang-ming~1, WANG Zhao-jie~2 (~1Department of Nephrology,?~2?Department of Pathology, Guangdong Provincial People's Hospital, Guangzhou 510080, China);Role of TGF-β_1 and its signaling transduction molecule Smad4 in the development of glomerulosclerosis[J];Chinese Journal of Pathophysiology;2005-07
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 GUO Xiao-li(College of Life Science,Hengshui University,Hengshui,Hebei 053000,China);Studies on the Growth Appearance of E.coli DH5α[J];Journal of Hengshui University;2010-01
2 YANG Guo-rong, XUE Yong-jie, JIA Xing-min, HE Xiao-xia, HE Xue-jiao, YU Rong Department of Pathology, The First Hospital of PLA, Lanzhou 730030, China;Role of decorin in liver fibrosis[J];Chinese Hepatology;2007-06
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