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《Acta Agriculturae Boreali-occidentalis Sinica》 2001-01
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Study on the DNA Mutation of Wheat Aphid under the Ultraviolet

YAO Jian xiu, ZHAO Hui yan (College of Plant Protection, Northwest Sci Tech University of Agriculture and Forestry, Yangling Shaanxi 712100)  
The DNA mutation rate of Macroiphum avenae under the ultraviolet was studied by the method of random polymorphic amplified DNA (RAPD PCR), and the method of cluster analysis based on the data of similarity index and Nei′s genetic distance. The result indicated that four primers(s88、s71、s103、s66) can be used for evaluating in the total clones, and that the average genetic distance equaled to 0.2028 under the ultraviolet (2×40 W), but it was 0.08125 under the normal condition. Therefore, the net genetic distance of the aphid under ultraviolet was 0.12155, and on this level, the rate of DNA mutation was about 47.6667%. Though we can conclude that the ultraviolet can result in mutation of the aphid DNA, whether this mutation has heredity remains to further study.
【Fund】: 国家自然科学基金! (39970 112 ) ;; 杨陵生物育种专项基金资助
【CateGory Index】: S433
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