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《Acta Agriculturae Boreali-Occidentalis Sinica》 2008-06
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Analyses on mtDNA D-loop Genetic Diversity in Guanzhong Horse

SU Rui1,XIE Wen-mei1,ZHANG Yun-sheng1,WANG Xiao-bin1,ZHANG Chun-mei1, WANG Mei-ju1,CHEN Hong1,2 and LEI Chu-zhao1(1.College of Animal Science and Technology,Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling Shaanxi 712100,China;2.Institute of Cellular and Molecular Biology,Xuzhou Normal University,Xuzhou Jiangsu 221116,China)  
The genetic diversity and phylogenetic evolution of 247 bp mitochondrial DNA(mtDNA) D-loop sequences of 27 Guanzhong horse samples were analyzed by sequencing and bioinformation techniques.The results revealed 9 mitochondrial haplotypes with 19 polymorphic sites,covering 7.69% of the analyzed length of the 247 bp D-loop sequences.Among the polymorphic sites,there were all transitions.The nucleotide diversity(π value) and haplotype diversity(H) estimated were 0.0219±0.0076 and 0.843±0.041,respectively,showing high mitochondrial genetic diversity in Guanzhong horse breed.The constructed Neighbor-Joining molecular phylogenetic tree based on the 9 mtDNA D-loop haplotypes,indicated that lineages A,C,D,F and G were detected which showed multiple maternal origins in Guanzhong horse breed.
【Fund】: 教育部新世纪优秀人才支持计划(NCET-07-0699);; 西北农林科技大学科研专项(07ZR004)
【CateGory Index】: S821
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