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《Acta Agriculturae Boreali-Occidentalis Sinica》 2010-02
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Development and Trait Identification of Colloidal Gold-Labeled Strip for Rapid Detection of Streptomycin

FAN Guoying1,CHEN Yongyao1,WANG Shungang1,YANG Yanyan2,DENG Runguang2,YANG Jifei2 and ZHANG Gaiping2(1.The Animal Science College,He′nan Institute of Science and Technology,Xinxiang He′nan 453003,China;2.Key Laboratory for Animal Immunology,He′nan Academy of Agricultural Science,Zhengzhou 450002,China)  
Based on the generation of monoclonal antibody(mAb) against Streptomycin(SM),the rapid test strip of SM was developed and its sensitivity,specificity,repeatability and stability were tested respectively.The strip showed that its detection limit was 8 μg/L,it generally had upper 100 μg/L;The strip has 100% cross-reactivity towards dihydrostreptomycin and little or no cross-reactivity towards other antibiotics.It′s sensitivity was as same as that of ELISA-Kit and its coincidence rate was 100% compared with ELISA-Kit.With the strip,the test results were showed in less than 10min,apart from very short time to get test results.
【Fund】: “十一五”国家科技支撑计划重大项目(2006BAK02A21)
【CateGory Index】: S859.84
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