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《Acta Agriculturae Boreali-Occidentalis Sinica》 2011-03
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Characterization and Application of EST-SSR Markers in Tea Plants(Camellia sinensis(L.) O.Kuntze)

LIU Benying,SUN Xuemei,JIANG Huibing,LI Youyong,SONG Weixi,MA Ling,YI Bing,WANG Yungang and WANG Pingsheng (Tea Research Institute of Yunnan Academy of Agricultural Science,Menghai Yunnan 666201,China)  
The frequency and characteristics of SSR were analyzed and clarified in ESTs of tea plant(Camellia sinensis(L.) O.Kuntze).3 306 ESTs of tea plant were downloaded from the public database of NCBI,among which the redundant or low quality were removed,finally obtained 1335 non-redundant ESTs with total length of 635.46 kb.Totally 216 SSRs distributed in 185 ESTs were found,accounting for 16.18% of the non-redundant ESTs,and the average distance of distribution is 1/2.94 kb.EST-SSR types in tea plant are considerably abundant,among which the dinucleotide repeat is the dominant type.These results provide a base for the development and application of EST-SSR markers in tea plants.According to tea ESTs,60 SSR primer pairs form 100 tea EST-SSRs were designed with Primer 5.0 software.After tested on the annealing temperature and polymorphism of primers,30 of them produced stable and clear bands with better polymorphism in 33 tea germplasms.A total of 71 putative alleles were generated,and ranged from 2 to 8,with a mean of 4.9 for putative alleles per locus.The polymorphism information content(PIC) varied from 0.00 to 0.731and the observed heterozygosity(Ho) ranged from 0.00 to 0.970,with an average of 0.499 and 0.392,respectively.All the tested SSR primers showed a high level of genetic polymorphism.
【Fund】: 云南省农科院茶叶所所列项目(2009A0937);; 国家现代农业产业技术体系(茶叶)建设项目;; 云南省自然科学基金重点项目(2006C0012Z)
【CateGory Index】: S571.1
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【Citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Liang 1,2 ,YU Fu lian 2,TONG Qi qing 1 [WT6BX](1 Department of Tea Science, Zhejiang University, Hangzhou 310029, China; 2 Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China )[WT5”HZ];Discussions on Phylogenetic Classification and Evolution of Sect Thea[J];JOURNAL OF TEA SCIENCE;2000-02
2 JIN Ji-qiang1,CUI Hai-rui1*,CHEN Wen-yue2,LU Mei-zhen1,YAO Yan-ling1,XIN Ya2,GONG Xiao-chun1(1.Institute of Nuclear and Agricultural Sciences,Zhejiang University,Hangzhou 310029,China;2.Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China);Data Mining for SSRs in ESTs and Development of EST-SSR Marker in Tea Plant (Camellia sinensis)[J];Journal of Tea Science;2006-01
3 YANG Yang,LIU Zhen,ZHAO Yang,LIANG Guo-qiang,ZHAO Xi(Tea Research Institute,Hunan Academy of Agriculture Science,Changsha,410125,China);Genetic Diversity and Relationship of Huangjincha Cultivar Based on EST-SSR Markers[J];Journal of Tea Science;2009-03
4 YAO Ming-zhe1,2,LIU Zhen2,CHEN Liang2,WANG Xin-chao2,MA Chun-lei2,LIANG Yue-rong1(1.College of Agriculture and Biological Technology,Zhejiang University,Hangzhou 310029,China;2.National Center for Tea Improvement,Tea Research Institute Chinese Academy of Agricultural Sciences,Hangzhou 310008,China);Genetic Diversity and Structure of Tea Germplasm Originated from Region of North Yangtze River Based on EST-SSR Markers[J];Journal of Tea Science;2009-03
5 Liu Zhen 1 Wang Xinchao 1 Zhao Liping 1 Yao Mingzhe 1 Wang Pingsheng 2 Xu Mei 2 Tang Yichun 2 Chen Liang 1 1 Research Center for Tea Germplasm and Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Hangzhou, 310008; 2 Tea Research Institute of Yunnan Academy of Agricultural Sciences, Menghai, 666201;Genetic Diversity and Relationship Analysis of Tea Germplasms Originated from South Western China Based on EST-SSR[J];Molecular Plant Breeding;2008-01
6 DONG Yuan-yuan YU Mi-na LI Xiao-bai XU Pan-feng CUI Hai-rui ZHANG Ming-long(Institute of Nuclear and Agricultural Sciences/Nuclear Agricultural Sciences key laboratory of Agricultural Ministry,Zhejiang University,Hangzhou,Zhejiang 310029);DIVERSITY ANALYSIS OF Brassica napus BY EST-SSR AND RAPD MARKERS[J];Journal of Nuclear Agricultural Sciences;2008-05
7 JIAO Deli,XU Liang,YAO Yanmei and DU Dezhi*(Qinghai University,Xining Qinghai 810016,China);Genetic Diversity Analysis of Doubled Haploid Population in Rapeseed(Brassica napus L.) Based on SSR Markers[J];Acta Agriculturae Boreali-Occidentalis Sinica;2009-01
8 JIN Ji-Qiang1, CUI Hai-Rui1, GONG Xiao-Chun1 ,CHEN Wen-Yue2, XIN Ya2 1. Institute of Nuclear and Agricultural Sciences, Zhejiang University, Hangzhou 310029, China; 2. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China;Studies on tea plants (Camellia sinensis) germplasms using EST-SSR marker[J];Hereditas;2007-01
9 MING Tien-Lu (Kunming Institute of Botany, Academia Sinica.Kunming 650204);A REVISION OF CAMELLIA SECT. THEA[J];Acta Botanica Yunnanica;1992-02
10 Chang Hung-ta;A REVISION ON THE TEA RESOURCE PLANTS[J];Acta Scientiarum Naturalium Universitatis Sunyatseni;1984-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Mei-yun et al(College of Landscape and Art of Jiangxi Agricultural University,Nanchang,Jiangxi 330045);Applications of Molecular Marker Technology in Tea[J];Journal of Anhui Agricultural Sciences;2008-11
2 LEI Yan-fang1,WEI Zhen-wu2*,YANG Zhan-hua1,GAI Jun-yi3,WANG Yi-ming1(1.Pratacultural College,Gansu Agricultural University,Lanzhou,Gansu Province 730070,China;2.College of Animal Science & Technology,Yangzhou University,Yangzhou,Jiangsu Province 225009,China;3.National Center for Soybean Improvement,Nanjing Agricultural University,Nanjing,Jiangsu Province 210095,China);Genetic Analysis of Seed Yield Characters in Medicago truncatula Gaertn.[J];Acta Agrestia Sinica;2009-03
3 XIONG Changyun (Tea science department of Yunnan agriculture university Kunmin 650201);Application of HACCP to black tea production in yunnan province[J];Journal of Tea;2004-02
4 TANG Yichun1,2,YAN Shengmei2,XU Mei2,WANG Pingsheng2,SONG Weixi2,YI Bing2(1.College of Long Run Pu-er Tea Y.A.U,Yunnan,Kunming 650201;2.Tea Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Menghai 666200);The protection and rational utilization of Yunnan big-leaf tea resource[J];Journal of Tea;2007-04
5 Du Qizheng;Li Mingjun;Liu Wehua(Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou)Wang Haisi(Tea Research Institute,Yunnan Academy of Agricultural Sciences,Monghai);Chemical and Numerical Taxonimies of Plants Thea Section Plants[J];Journal of Tea Science;1990-02
6 Liang Guolu;Li Xiaoli;Wang Suoshen;Zhou Caiqiong(Southwest Agricultural University,Chongqing630716);Analysis on Karyotypes of Sect.Thea in Sichuan[J];Journal of Tea Science;1993-02
7 CHEN Liang 1,2 ,YU Fu lian 2,TONG Qi qing 1 [WT6BX](1 Department of Tea Science, Zhejiang University, Hangzhou 310029, China; 2 Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China )[WT5”HZ];Discussions on Phylogenetic Classification and Evolution of Sect Thea[J];JOURNAL OF TEA SCIENCE;2000-02
8 LI Xing_hui 1, SHI Zhao_peng 1, LIU Chun_lin 2, LUO Jun_wu 1, SHEN Cheng_wen 1, GONG Zhi_hua 1 (1. Hunan Agricultural University, Changsha 410128, China; 2. Crop Gene Engineering Key Laboratory of Hunan Province, Changsha 410128, China);Parentage Identification of Filial Generation Tea Plants from "Yunnan Daye" and "Rucheng Baimao" with RAPD Method[J];Journal of Tea Science;2001-02
9 CHEN Liang1,2, YAMAGUCHI Satoshi3, WANG Ping-sheng4, XU Mei4, SONG Wei-xi4, TONG Qi-qing1 (1.Department of Tea Science, Zhejiang University, Hangzhou 310029, China;2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;Genetic Polymorphism and Molecular Phylogeny Analysis of Section Thea Based on RAPD Markers[J];Journal of Tea Science;2002-01
10 LI Xing-hui1,SHI Zhao-peng1,LIU Chun-lin2,LUO Jun-wu1,SHEN Cheng-wen1,LI Jia-xian3,FANG Hua-chun3 (1. Hunan Agricultural University, Changsha 410128, China;2. Crop Gene Engineering Key Lab. of Hunan Province, Changsha 410128, China; 3. Tea Research Instit;The Genetic Relationships of“ucheng Baimao Cha?and the Main Populations of ”aimao Cha?in Guangdong and Guangxi Region with RAPD Method[J];Journal of Tea Science;2002-01
China Proceedings of conference Full-text Database 9 Hits
1 TANG Yi-chun,Xu Mei, WANG Ping-sheng, SONG Wei-xi (Tea Research Institute Yunnan Academy of Agricultural Sciences, Yunnan Menghai 666200);Preliminary Study on Innovative Utilization of Wild Tea Germplasm Resources[A];[C];2007
2 ;Genetic Diversity of C. taliensis from Yunnan Province of China Revealed by AFLP Analysis[A];[C];2009
3 Qiao Ting-ting, Ma Chun-lei, Zhou Yan-hua, Yao Ming-zhe, Chen Liang* Research Center for Tea Germplasm and Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences; National Center for Tea Improvement, Hangzhou 310008, China;EST-SSR Analysis of Genetic Diversity and Structure of Tea Landraces and Bred Cultivars in Zhejiang Province,China[A];[C];2009
4 TANG Yi-chun1, YAN Sheng-mei1,SONG Wei-xi1,MA Ling1,WANG Yun-gang1,CHENG Hao2 (1 Tea Research Institute of Yunnan Academy of Agricultural Science, Yunnan Menghai 666200,China;2Tea Research Institute of Chinese Academy of Agricultural Science,Hangzhou 310008,China);Preliminary Studies on Pollen Viability of Sect. Thea and Hybrids of Species[A];[C];2009
5 Wang Xin-Chao, Chen Liang*, Yang Ya-Jun*, Yao Ming-Zhe, Ma Chun-Lei (Research Center for Tea Germplasm and Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences; National Center for Tea Improvement, Hangzhou 310008, China);Establishment of a Primary Core Collection of Tea Plant Based on Cultivated Region Grouping and Phenotypic Data in China[A];[C];2009
6 YANG Yang, LIU Zhen, ZHAO Yang, YANG Pei-di (Tea Research Institute, Hunan Academy of Agriculture Science, Changsha, 410125, China);Construction of DNA Fingerprint for Tea Cultivars Originated from Hunan[A];[C];2009
7 YAO Mingzhe,QIAO Tingting,MA Chunlei, JIN Jiqiang,CHEN Liang*,YANG Yajun* (Tea Research Institute Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Hangzhou 310008, China);The Association Analysis of Phenotypic Traits with EST-SSR Markers in Tea Plants[A];[C];2009
8 ZHANG Shu-guang, DONG Li-juan, YANG Yang, LIU Zhen, WANG Xu, NING Jing (Tea Research Institute, Hunan Academy of Agriculture Science, Changsha 410125, China);Identification of Male Parents for “Yulv” and “Yusun” Tea Variety Based on EST-SSR Markers[A];[C];2009
9 Xiaoli zhou~1,Chunsheng Liu~1,Xueyong Wang~1,Luqi Huang~2 (1.Beijing university of Chinese medicine,Beijing,100102;2.Institute of Chinese materia medica,China academy of Chinese medical sciences,Beijing,100700);Study of EST-SSR in medical plants[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhang Li et al (Beijing Vegetable Research Center, Beijing 100089);Progress of Haploid Breeding in Cruciferae Crops[J];Journal of Anhui Agricultural Sciences;2003-02
2 Tan Yongji;Chen Binghuan;Yu Fulian(Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou)Wang Haisi;Wang Pingsheng(Tea Research Institute,Yunnan Provincial Academy of Agricultural Sciences,Menghai);New Species & Variety of Tea Plants Found in Yunnan,China[J];Journal of Tea Science;1984-01
3 Yu Fulian;Discussion on the Originating Place and the Originating Center of Tea Plant[J];Journal of Tea Science;1986-01
4 Li Bin;Chen Xingyan;Chen Guoben;Wang Jianguo(Tea Laboratory of Hunan Agricultural College,Changsha);The Analysis of Karyotype in Tea Plant[J];Journal of Tea Science;1986-02
5 Du Qizheng;Li Mingjun;Liu Wehua(Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou)Wang Haisi(Tea Research Institute,Yunnan Academy of Agricultural Sciences,Monghai);Chemical and Numerical Taxonimies of Plants Thea Section Plants[J];Journal of Tea Science;1990-02
6 KIM Hyesuk,LIANG Yue_rong,LU Jian_liang (Department of Tea Science, Zhejiang University, Hangzhou 310029, China);Comparative Study on Genomic DNA Diversity between Korean and Chinese Tea Cultivars by RAPD Technique[J];Journal of Tea Science;2001-02
7 WANG Li-yuan, CHENG Hao, ZHOU Jian (Key Lab of Tea Chemical Engineering, Ministry of Agriculture; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China);Advances on DNA Molecular Markers and Gene-engineering in Tea Plants[J];Journal of Tea Science;2004-01
8 HUANG Fu-ping1,2, LIANG Yue-rong1, LU Jian-liang1, CHEN Rong-bing2, Mamati G. E.1, SUN Qing-lei1 (1. Tea Research Institute, Zhejiang University, HangZhou 310029, China; 2. Tea Research Institute, Fujian Acaedemy of Agricultrual Sciences, Fu’an 355000, China);Evaluation of Genetic Diversity in Oolong Tea Germplasms by AFLP Fingerprinting[J];Journal of Tea Science;2004-03
9 JIN Ji-qiang1,CUI Hai-rui1*,CHEN Wen-yue2,LU Mei-zhen1,YAO Yan-ling1,XIN Ya2,GONG Xiao-chun1(1.Institute of Nuclear and Agricultural Sciences,Zhejiang University,Hangzhou 310029,China;2.Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China);Data Mining for SSRs in ESTs and Development of EST-SSR Marker in Tea Plant (Camellia sinensis)[J];Journal of Tea Science;2006-01
10 Chen Liang 1, Chen Daming 2, Gao Qikang 2, Yang Yajun 1, Yu Fulian 1 (1 Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008; 2 Zhejiang Agricultural University, Hangzhou 310029);Isolation and Appraisal of Genomic DNA From Tea Plant [ Camellia sinensis (L.)O.Kuntze][J];JOURNAL OF TEA SCIENCE;1997-02
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