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《Acta Agriculturae Boreali-Occidentalis Sinica》 2011-03
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Characterization and Application of EST-SSR Markers in Tea Plants(Camellia sinensis(L.) O.Kuntze)

LIU Benying,SUN Xuemei,JIANG Huibing,LI Youyong,SONG Weixi,MA Ling,YI Bing,WANG Yungang and WANG Pingsheng (Tea Research Institute of Yunnan Academy of Agricultural Science,Menghai Yunnan 666201,China)  
The frequency and characteristics of SSR were analyzed and clarified in ESTs of tea plant(Camellia sinensis(L.) O.Kuntze).3 306 ESTs of tea plant were downloaded from the public database of NCBI,among which the redundant or low quality were removed,finally obtained 1335 non-redundant ESTs with total length of 635.46 kb.Totally 216 SSRs distributed in 185 ESTs were found,accounting for 16.18% of the non-redundant ESTs,and the average distance of distribution is 1/2.94 kb.EST-SSR types in tea plant are considerably abundant,among which the dinucleotide repeat is the dominant type.These results provide a base for the development and application of EST-SSR markers in tea plants.According to tea ESTs,60 SSR primer pairs form 100 tea EST-SSRs were designed with Primer 5.0 software.After tested on the annealing temperature and polymorphism of primers,30 of them produced stable and clear bands with better polymorphism in 33 tea germplasms.A total of 71 putative alleles were generated,and ranged from 2 to 8,with a mean of 4.9 for putative alleles per locus.The polymorphism information content(PIC) varied from 0.00 to 0.731and the observed heterozygosity(Ho) ranged from 0.00 to 0.970,with an average of 0.499 and 0.392,respectively.All the tested SSR primers showed a high level of genetic polymorphism.
【Fund】: 云南省农科院茶叶所所列项目(2009A0937);; 国家现代农业产业技术体系(茶叶)建设项目;; 云南省自然科学基金重点项目(2006C0012Z)
【CateGory Index】: S571.1
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