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《Acta Agriculturae Boreali-Occidentalis Sinica》 2011-04
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Phylogeny of Eupatorium adenophorum Spreng in Asteracea Basing on Chloroplast Sequences

TAN Xiufang1,DU Xianghong1,NIU Shance1,NIE Xiaojun1,ZHANG Yingxin1,WAN Fanghao2 and SONG Weining1(1.College of Agronomy,Northwest A &F University,Yangling Shaanxi 712100,China;2.Center for Management of Invasive Alien Species,Ministry of Agriculture,Institute of Environment and Sustainable Development in Agriculture,CAAS,Beijing 100081,China)  
In order to analyze the taxonomic status of Eupatorium adenophorum Spreng in Asteracea,the chloroplast rpl16,psbA-trnH and trnL-F of Eupatorium adenophorum Spreng were amplified and sequenced.All the sequences were analyzed basing on sequence alignment with other chloroplast genes of Asteracea species in GenBank.Phylogenetic analysis was conducted with Mega 4.0 software.Phylogenetic relationships were estimated by neighbor-joining method.The results indicated that,The Composite plants can be classified as two classes in the phylogenetic systematic.The class contained Chrysanthemum indicum and Leucanthemum vulgare was far related to Eupatorium adenophorum Spreng;Eupatorium adenophorum Spreng had the closest taxonomic status with other plants of the genus Eupatorium and Guizotia abyssinica,and it was much closer than that between Helianthus annuus and Parthenium argentatum and the relation between Eupatorium adenophorum Spreng and Lactuca sativa,Leucanthemum vulgare and Chrysanthemum indicum was far.So our molecular evidences indicate that Eupatorium adenophorum Spreng belongs to the genus Eupatorium;The sequences of the combined dataset(rpl16 + psbA-trnH + trnL-F)ranged from 2 140 bp to 2 203 bp in length.The sequences were aligned and final sequences were 2 399 bp in length,including 381 variable sites and 190 parsim-info sites.Analyses of three separate and combined datasets provided a good amount of informative characters and resolved the systematical relationships of Eupatorium adenophorum Spreng well.
【Fund】: 重要外来物种入侵的生态影响机制与监控基础(973计划)(2009CB119201)
【CateGory Index】: S451
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