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《Acta Agriculturae Boreali-Occidentalis Sinica》 2012-07
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Establishment of Testicle Fibroblast Line and Its Biological Characteristics in Hequ Horse

LING Shixin1,GUAN Weijun2,FENG Yuping3,QIAO Zilin1,FENG Ruofei1, LI Mingsheng1,WANG Jiamin3,SHENG Wuling3 and MA Zhongren1(1.Gansu Engineering Research Center for Animal Cell,Lanzhou 730030,China; 2.Insitute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China; 3.College of Life Science and Engineering,Northwest University for Nationalities,Lanzhou 730030,China)  
The testical explants of Hequ horse were cultured with trypsinization.The primary cells were purified and preserved with liquid nitrogen after three generations.Observations on morphology,dynamic growth,microbial contamination,analysis of karyotype,isoenzyme of lactate dehydrogenase and fluorescin plasmid transfection and expression were carried out.The results showed that the cell line was fibroblast and grew well.The maximum proliferation density was 4.32×105 mL-1 and population doubling time of cells(PDT) was 34.58 h;the tests for bacteria,fungi,virus and mycoplasma were all negative;the chromosomes were 64 and diploid cells were dominant of 85%.The banding patterns of the isoenzyme of lactate dehydrogenase were significant characteristics.There were four bands in all.As follow,the LDH3 was higher and stronger.The exogenous plasmid could copy and express in the cell.Those results showed that the cell line was established successfully,which makes the Hequ horse,a national important genetic resource preserved in cell level,as well as will provide an ideal experimental material for genetic studies.
【Fund】: 国家自然科技资源共享平台项目(2005DKA21101)
【CateGory Index】: S821.84
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