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《Acta Agriculturae Boreali-Occidentalis Sinica》 2014-01
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Analysis on Antioxidant Enzyme Activities and Peroxidase Isozymes in 23 Rape Cultivars

SHI Penghui;LIU Zigang;ZHANG Yahong;SUN Wancang;KONG Dejing;LU Meihong;YANG Ningning;Gansu Key Lab of Crop Improvement & Germplasm Enhancement,Gansu Provincial Key Laboratory of Aridland Crop Sciences,College of Agronomy,Gansu Agricultural University;Tianshui Institute of Agriculture;  
Antioxidant enzyme activities(SOD,CAT and POD)and the Omolality of solute MDA of 23 rape cultivars were measured,and the peroxidase isozymes of 23samples were studied by polyacrylamide gel electrophoresis analysis to identify the characteristics and distribution of the enzyme spectrum. The genetic relationship among these cultivars was studied with cluster analysis method.The results showed that,in mild conditions,compared to the Brassica napus,Brassica campestris had higher antioxidant enzyme activity and a lower level of lipid peroxidation.It indicated that Brassicacampestris had strong stress resistance;especially average peroxidase activity was 10times stronger than that of Brassica napus.67bands were found by POD isozyme analysis,and Brassica campestris and Brassica napus has its own characteristic bands,there are also differences in the band patterns and color intensity in different varieties.In Brassica campestris Largo,Prisma-B and Prisma-Y,its p4enzyme band are consistent with Brassica napus,which indicates that there is a close genetic relationship betweenthese materials and Brassica napus.The cluster analysis showed that 23rape samples were divided into two categories(Brassica campestris and Brassica napus)at the average class distance of 4.98.It can be further divided into four categories at the average class distance of 1.32.
【Fund】: 农业部公益性行业(农业)科研专项经费项目(200903002-04);; 国家高技术研究发展计划(863计划)项目“强优势油菜杂交种的创制与应用(2011AA10A104)”;; 现代农业产业技术体系建设专项资金(CARS-13)
【CateGory Index】: S565.4
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