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《Journal of Northwest Sci-Tech University of Agriculture and Forestry》 2001-05
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Study on immunogenicity of swainsonine-BSA

TONG De wen,CHEN De kun,CAO Guang rong (College of Animal Sciences and Veterinary Medicine,Northwest Sci Tech University of Agiculture and Forestry,Yangling,Shaanxi 712100,China)  
After basically immunized with complete Freunds adjuvant,14 rabbits were divided into 2 groups equally.Group one was immunized with swainsonine BSA,which was used as the vaccine after emulsifying it with complete Freunds adjuvant.The other was the control gruop.Immune procedure was as followings.The rabbit was injected 2 mL swainsonine BSA vaccine,containing 4 mg swainsonine BSA.0.8 mL was injected into 4 plantae,each fore plantae was 0.15 mL and hind plantae was 0.25 mL.0.6 mL was injected inside hind leg,with each leg 0.3 mL,i.m.0.6 mL was injected into neck,i.p.After 5 weeks the first intensive immunization was conducted,swainsonine BSA was dissolved in water for injection.2 mL of the solution,containing 6 mg swainsonine BSA,was injected to the rabbit.1 mL was injected inside hind leg,each leg 0.3 mL,i.m.1 mL was injected 4 points of back,0.25 mL in each point.Antibody was monitored after a week.The second intensive immunization was conducted after 10 days.Immune dose was 2 mL,containing 12 mg swainsonine BSA.Immune way was the same as the first intensive immunization.Antibody was monitored after a week.Immunoassay was conducted with indirect hemagglutination test and indirect ELISA test.After a week of the intensive immunization,all rabbits produced antibody.The titres,monitored by indirect hemagglutination test,were 2 1,2 1,2 2,2 1,2 1,2 2 and 2 2,and indirect ELISA test,were 2 2,2 2,2 3,2 2,2 2,2 3 and 2 3.After a week of the second intensive immunization,the titres,monitored by indirect hemagglutination test,were 2 6,2 6,2 8,2 7,2 7,2 8 and 2 8,and indirect ELISA test,were 2 7,2 7,2 9,2 8,2 8,2 9 and 2 9.
【Fund】: 国际科学基金资助项目 ( IFS;;No2 90 6 -1) ;; 国家自然科学基金资助项目 ( 39770 5 71)
【CateGory Index】: S859.87
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