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《Journal of Northwest Sci-Tech University of Agriculture and Forestry》 2004-01
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Molecular markers of the fertility genes Rf_3 and rfk_1 in the 1BS chromosome of T.Spelta

HU Yin-gang~1,MA Ling-jian~1,SONG Xi-yue~1,FANG Peng~1,HE Pei-ru~1,ZHANG Wen-jun~2 (1 Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China; 2 PCCE Lab,Institute of Genetics,Chinese Academy of Sciences,Beijing 100101,China)  
To breed non 1B/1R K type male sterile wheat lines,He Peiru designed a chromosome transfer method to transfer the fertility fragments in the 1Bs of T.Spelta (which including Rf_3 and rfk_1 genes) to common wheat,during this process,Rf_3 gene was used as the marker of the present of rfk_1 gene under the backgroud of T.timophvee cytoplasm.However,exchanges of the chromosome fragments were observed during the transformation progress,some materials were fertile male under the T.timophvee cytoplasm but did not carry the rfk_1 gene with them,it is necessary to study the molecular marker for the fertility fragments in the 1Bs of T.Spelta,which could be used in marker-assisted breeding of the non 1B/1R K type male sterile wheat lines.AFLP was used in the maker analysis of the fertility genes Rf_3 and rfk_1,which are located in the 1Bs chromosome of T.Spelta,the results showed that two primer combinations P3-T2 and P4-T3 could give 4 very good polymorphism bands respectively to the Rf_3 and rfk_1 genes based on the fertility of the F_2 individuals under both of the T.timophvee and Aegilops kotschyi cytoplasm.
【Fund】: 国家863计划基础研究项目(2001AA241043)
【CateGory Index】: S512.103.53
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