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《Journal of Northwest Sci-Tech University of Agriculture and Forestry》 2004-05
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Segregation and culture of endothelial cells from swine umbilicus veins

HONG Hai-xia,ZHANG Yan-ming,SUN Pei,SU Zheng-yuan,WEI Zhong-feng,ZHANG Yong-guo (College of Animal Science and Technology,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China)  
Swine umbilical cord was sterilized by 75% ethyl alcohol about 1 min and umbilicus veins were washed by PBS.1 g/L callagenase Ⅰ was poured into umbilicus vein and digested 5 min at 37 ℃.Endothelial cells obtained was collected by centrifugation,cultured in M199+20% fetal calf serum.The plating efficiency to plastic was 70%-75% for 24 hours in callagenase dispersed cells.A confluent monolayer was former by 4-5 days.The the endothelial cells looked like cobblestones and were stained positively for factor Ⅷ relative antigen,which is feature of endothelial cells.It shows cells cultured are umbilicus veins cells.
【Fund】: 国家自然科学基金项目(30270988)
【CateGory Index】: Q954.6-332
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