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《Journal of Northwest Sci-Tech University of Agriculture and Forestry》 2005-10
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Comparison of total RNA content in different organs of wheat by different methods

YU Xiu-mei~a,KANG Zhen-sheng~b,CUI Su-ping~a,ZHAO Jie~b(a College of Plant Protection,b College of Life Sciences,Northwest A & F University,Yangling,Shaanxi 712100,China)  
Total RNA was extracted from young leaf,young stem,radicle,and immature seed of wheat by four different methods respectively.The results showed that all of the purified total RNA were up to standard and general downstream applications through purity and quantity analysis.Among these methods,TRIzol could get the highest production of total RNA,which was as much as 2-4 times that of other methods.Tianweishidai kit needed less time,and could operate at room temperature.Due to fewer steps,less time,lower price and suitable for large quantity samples,NaAC/ethanol was a desirable method to extract total RNA from different organs in wheat.Detected by Ultraviolet spectrophotometer,A_(260)/A_(280) was 1.88-2.09 and A_(260)/A_(230) was 1.89-2.41,which showed the purity of total RNA was better.Analyzed by formaldehyde denaturalized gel electrophoresis,total wheat RNA contained large percentage of chloroplast rRNA other than cytoplasm rRNA,which resulted in two more rRNA bands in young leaf and young stem than those in radicle.
【Fund】: 教育部高校科技创新工程重大项目培养资金
【CateGory Index】: S512.1
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