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《Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition)》 2006-12
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Two efficient improved methods for isolation of high quality total RNA from soybean

LIU Yi-ke~(1,2),SUN Hong-bo~2,JIAN Bo~2,HU Po~2,GAO Xiao-wei~2,HOU Wen-sheng~(1,2)(1 College of Agronomy,Northwest A & F University,Yangling,Shaanxi 712100,China;2 Crop Science Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)  
Two methods of isolating RNA were improved in this paper.The effects of extracting total soybean RNA with the improved methods were compared with those original ones.The result shows the improved Trizol method fits for extracting RNA from soybean root,stem,leaf and shoot apical mer,whereas the improved Guanidinium Thiocyanate method fits for root,stem and shoot apical meristem(SAM).More than 22 μg RNA was obtained from each 100 mg plant tissue with high purity and integrity.Therefore,these methods deserve further studies.
【Fund】: 国家自然科学基金项目(30471054)
【CateGory Index】: S565.1
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【References】
Chinese Journal Full-text Database 8 Hits
1 Zhou Xianghong;Wang Ping;School of Marine Science & Technology,HuaiHai Institute of Technology;;Simultaneous Isolation of RNA and DNA from Soybean Cotyledon and Analysis of Their Quality[J];作物杂志;2013-06
2 Guo Bin 1,2 Hou Siyu 1 Huang Kesheng 1,2 Lu Yang 1 Han Yuanhuai 1 Wang Yuguo 2* 1 Institute of Agricultural Bioengineerings, Taigu, 030801; 2 School of Agriculture, Shanxi Agricultural University, Taigu, 030801;Different Methods for Extracting Total RNA and Their Application in Gene Cloning and Gene Expression Analysis in Soybean (Glycine max)[J];分子植物育种;2013-02
3 Mo Beixin1,Huang Biao1,Liu Li2,Ye Hao1,and Xu Xiaofeng2 1)College of Life Science,Shenzhen Key Laboratory of Microbial Genetic Engineering,Shenzhen University, Shenzhen 518060,P.R.China 2) College of Life Sciences,Shenzhen Key Laboratory of Marine Biological Resources and Ecological Environment, Shenzhen University,Shenzhen 518060,P.R.China;Utilization of stored mRNA during the germination of soybean radicle[J];深圳大学学报(理工版);2013-01
4 WU Zhi-yan,YE Jing-yun,CHENG Shan,YAN Xun-you(College of Life Science,Langfang Teachers College,Langfang 065000,China);Comparison of the RNA isolation methods for Pleurotus ferulae Lenzi hyphostroma[J];浙江农业学报;2012-06
5 FENG Yan-zhi,YUAN De-yi,ZHANG Lin,LONG Hong-xu,XU Jing-shi,LIU Min(The Key Lab.of Non-wood Forest Product of Forestry Ministry,Central South University of Forestry & Technology,Changsha 410004,Hunan,China);Comparison of different extraction methods of total RNA from Ziziphus jujuba flower bud[J];经济林研究;2012-02
6 Chen Gao 1,2 , Shan Lei 2 , Zhou Lixia 1,2 , Tang Guiying 2 , Bi Yuping 1,2 ( 1 Science College, Shandong Normal Unveristy, Jinan 250014; 2 High-Tech Research Center, Shandong Academy of Agricultural Science, Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100);The Comparison of Different Methods for Isolating Total RNA from Peanuts[J];中国农学通报;2011-01
7 Yao Yingqiu1 Chen Xing2 Wang Pu1 Liang Yan2 Yan Mei2 Xiao Bai2(1College of Life Science,Capital Normal University,Beijing 100037;2Beijing Chaoyang Hospital Affiliate of Capital of Medical Sciences,Beijing 100020);Improved Methods for Isolation of High Quality Total RNA from Glycine max Leaves[J];生物技术通报;2008-01
8 Dai Yongxin Yang Xiangkun Wang Lin(Department of Horticulture,College of Agriculture,Shihezi University,Xinjiang 832003);Methods for Rapidly Extracting Total RNA from Stone Fruits[J];安徽农学通报;2007-07
【Citations】
Chinese Journal Full-text Database 6 Hits
1 WANG You hua 1,LU Meng zhu 2,DUAN Liu sheng 1 (1 College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094,China;2 Laboratory of Biotechnology,Institute of Chinese Forestry Academy,Beijing 100091,China);Modified Hot Phenol Method for Extracting Total RNA from Seedling Root of Cotton[J];西北植物学报;2005-04
2 Fu Chang Wang Yu-Ying Dai Hong-Jie (Life and Environment College, Harbin Normal University, Harbin, 150080);ANALYSIS OF RNA LATION FROM DIFFERENT SOYBEAN SRGAS[J];大豆科学;2004-04
3 SHI Gong-jun~1 ,HOU Xi-lin~(1,2) and YI Jin-xin~1 10095,China; 2. College of Horticulture, Nanjing Agric. Univ., Nanjing 210095,China);RNA Isolation and Analysis from Anthers of Non-heading Chinese Cabbage[J];西北农业学报;2004-03
4 LIU Zhi,YANG Yong-hua College of Life Sciences,Nanjing University,Nanjing210093,China;Extraction of total RNA from Onosma paniculatum,a Chinese medical plant,by CTAB method[J];生物技术通讯;2004-04
5 WANG Fang1,2, ZHANG Bin2, SHAN Lei2, BI Yu-ping2, XU Ze-yong3(1. School of Life Sciences, Shandong Normal University, Jinan 250014,China; 2. Plant Biology Technology Research Center, SAAS, Jinan 250100,China; 3. Oil Crops Research Institute ,CAAS,Wuhan 430062,China);An Improved Method for RNA Isolation from Peanut Seeds[J];花生学报;2002-04
6 Li Hong 1,2 Wang Xinli 1 1 Laboratory of Plant Biotechnology, Institute of Micobiology, Chinese Academy of Sciences, Bejing, 100080; 2 Department of Light Industry, Hebei Sciences and Technology University, Shijiazhuang, 050018;The Difficulties in the Isolation of RNA from Plant Tissues and their Resolving Strategies[J];生物技术通报;1999-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Chen Xiaofeng;Du Qiuli;Zhao Chunmei;Lv Zhaoyong;Xue Rengao;Qingdao Betterpet Foodstuff Co., Ltd.;Quancheng College, University of Jinan;College of Life Sciences,Qingdao Agricultural University;;Cloning and Expression Analysis of GmCYP78A5 Promoter[J];分子植物育种;2015-12
2 Kang Yaxuan;Chen Wu;Pang Xiaoming;Li Yingyue;National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plant, Ministry of Education, Beijing Forestry University;;The Comparison and Improvement of RNA Extraction Methods in Different Tissues in Chinese Jujube(Ziziphus jujuba Mill.)[J];分子植物育种;2015-12
3 XUAN Jiping;JIA Zhanhui;QIAN Meihua;ZHANG Jiyu;WANG Gang;GUO Zhongren;Zhongshan Botanical Garden,Institute of Botany,Chinese Academy of Sciences;;Comparative of Methods for RNA Extraction From Plum(Prunus salicina Lindl.)Fruit Flesh[J];北方园艺;2015-22
4 ZHANG Yan;HOU Kunlan;Life Science and Technology College,Yangtze Normal University;;Extracting RNA from Leaves of Mustard Brassica Juncea and Measurement and Analysis of Myrosinase Gene[J];长江蔬菜;2015-20
5 WU Ya-yun;ZHANG Shao-peng;ZHAO Xiao-long;SONG Jia;CHEN Ping;School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University;Lab of Molecular Pharmacology of Traditional Chinese Medicine in Hubei;;Analysis and optimization of high-quality RNA extraction in rhizomes of Panax japonicus C. A. Mey[J];武汉轻工大学学报;2015-03
6 ZHOU Yao-yao;CHEN Chun-yan;CHEN Peng;DIAO Yong;ZHOU Rui-yang;Provincial Key Laboratory of Plant Genetics and Breeding, Guangxi University;;A Method of Extracting High Quality Total RNA from the Anthers and Petals of Kenaf[J];天津农业科学;2015-09
7 LI An-yu;SHE Wen-qin;College of Horticulture,Fujian Agriculture and Forestry University;;Comparison of total RNA extraction methods of olive fruits[J];亚热带农业研究;2015-03
8 JIANG Suhua;LI Yanhui;WANG Jieqiong;LIANG Fang;WANG Mofei;CUI Bo;Institute of Biotechnology,Zhenzhou Normal University;Fengqiu Bureau of Agriculture;College of Life Science,Henan Agricultural University;;Comparison research of extraction method of total RNA from sedirea japonica[J];河南农业大学学报;2015-04
9 ZHANG Yuan-yuan;ZHAO Ji-qiang;JIANG Li-jun;SHI Yan-hui;CHEN Lei;GUO Shan-li;SONG Jian-cheng;Paula E Jameson;School of Life Sciences, Yantai University;School of Biological Sciences, University of Canterbury;;High Quality RNA Isolation from Mature Seeds of Maize Based on Modified and Optimized Protocols[J];玉米科学;2015-04
10 CHEN Chun-Yan;MA Hui-Ling;DONG Wen-Ke;College of Pratacultural Science,Gansu Agricultural University;College of Agronomy,Henan University of Science and Technology;Key Laboratory of Grassland Ecosystem,Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province;Sino-U. S. Center for Grazingland Ecosystem Sustainability;;Cloning and Expression Analysis of a Leucoanthocyanidin Reductase( LAR) Gene from Onobrychis viciifolia cv. Gansu[J];草业学报;2015-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 MO Bei-xin1,YE Hao1,OU Zhong-hua1,LIU Li1, HUANG Biao1,and ZHU Shuang-lin1,2 1) College of Life Science,Shenzhen University,Shenzhen 518060,P.R.China 2) Department of Health Science,McMaster University,Hamilton L8S4L8,Canada;Stress-induced processing body formation in tobacco suspension cells[J];深圳大学学报(理工版);2012-01
2 Xie Jingmei,Liu Xiaolan,Qu Cunmin,Lu Kun,Lv Jun,Li Jiana(Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University,Beibei 400716,Chongqing,China);Study of Rapid Methods for Isolation of DNA from the Seeds of Brassica napus L.[J];作物杂志;2012-01
3 REN Jie1,XU Xiu-hong1,ZHANG Su-li2,LENG Ping2(1.Key Laboratory of Tobacco Quality Control,Ministry of Agriculture,Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China; 2.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China);A Method for Effective Isolation of Total RNA from Different Plant Tissues[J];华北农学报;2011-S1
4 YI Le-fei1,2,LI Xin-shu2,YAN Bin-lun1(1.Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China; 2.School of Marine Science & Technology,Huaihai Institute of Technology,Lianyungang 222005,China);Comparison of Methods for Simultaneous Extraction of DNA and RNA from Seaweeds[J];水产科学;2011-11
5 XIAO Xu-feng,JIANG Wen-xuan,WU Cai-jun,FAN Shu-ying*(College of Agronomy,Jiangxi Agricultural Universiy,Nanchang 330045,China);Comparison of Different Methods for Total RNA Extraction from Pueraia Earthnut[J];江西农业大学学报;2011-01
6 LI Ji-dong1,WU Ying-xia1,FENG Jian-can1,BI Hui-tao1,LIANG Qi-wei1,KOU Shou-dao2(1.Henan Agricultural University,Zhengzhou 450002,Henan,China;2.Forestry Bureau of Xinzheng City,Xinzheng 451100,Henan,China);Compare of different extraction methods of RNA in Ziziphus jujuba[J];经济林研究;2010-04
7 Yu Limei Zhong Huizeng Yu Xin Zeng Xiaofang(College of Light Industry and Food Science,Zhongkai University of Agricultural andEngineering,Guangzhou 510225);Change of Nutritional Composition During Soybean Germination[J];中国粮油学报;2010-08
8 WEN Ya-feng,YUAN Qin-qin,HE Gang(Central South University of Forestry & Technology,Changsha 410004,Hunan,China);Advance in research on molecular breeding in Ziziphus jujuba Mill[J];经济林研究;2010-02
9 ZHANG YANG et al(Jilin Agriculture University,Changchun,Jilin 130118);Effects of Extraction RNA from Soybean Root under Different Developmental Stages[J];安徽农业科学;2010-17
10 SHI Yun-qing1,CHEN Gao2,SUN Bing2,SHAN Shi-hua1,CHEN Jing1,MIAO Hua-rong1,HU Xiao-hui1 (1.Shandong Peanut Research Institute,Qingdao 266100,China;2.Northeast Agricultural University,Haerbin 150030,China);Cloning and Sequence Analysis of NBS Class of Resistance Gene Homolog from Peanut Testa[J];山东农业科学;2009-12
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WEI Qi-qi;FENG Yan-zhi;LIN Qing;JIA Bao-guang;ZHANG Lin;The Key Lab of Non-wood Forest Products of State Forestry Administration, Central South University of Forestry & Technology;The Key Lab of Cultivation and Protection for Non-Wood Forest Trees of Education Ministry, Central South University of Forestry & Technology;2011 Cooperative Innovation Center of Cultivation and Utilization for Non-Wood Forest Trees of Hunan Province, Central South University of Forestry & Technology;The Center of Research and Development for Non-wood Forest Tree, Chinese Academy of Forestry;;Screening of total RNA extraction methods for RNA-sequences from different organs in Ziziphus jujuba[J];经济林研究;2015-02
2 ZHANG Xue;GUAN Li-li;HAN Yi-lai;YANG Jing;DU Lin-na;LI Xiao-kun;Ministry of Education Bioreactor and Drug Development Reseach Center,Jilin Agricultural University;College of Life Science,Jilin Agricultural University;;Study on Total RNA Extraction from Safflower Petals[J];黑龙江农业科学;2015-02
3 RONG Jing;RUAN Meng-bin;WANG Bin;PENG Ming;College of Agriculture,Hainan University;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Biology and Genetic Resources of Tropical Crops,Ministry of Agriculture;;Preliminary analysis on genetic characteristics and the promoter activity of MeMYB63 in cassava(Manihot esculenta Crantz)[J];广东农业科学;2014-17
4 Wen Suichao;Luo Jun;Wang Yinquan;Li Shunan;Gansu University of Traditional Chinese Medicine;Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province;;An Appropriate Method of Isolating Total RNA from Angelica sinensis for Quantitative RT-PCR[J];中国农学通报;2014-21
5 XU Hang;LIU Ren-xiang;XU Ru-hong;NIE Qiong;DUAN Xing-you;CHEN Shao-kai;WANG Guo-qin;YUAN Fu;CAI Lin;Guizhou University,Guizhou Key Laboratory of Tobacco Quality;;The Optimization of RNA Isolation and the Exploration of Ice Box Storage Time in Tobacco[J];江西农业大学学报;2014-03
6 LI Miao;LI Guo-qi;Key Lab for Restoration and Recovery of Degraded Ecosystem in Northwestern of Ministry of Education,Ningxia University;Key Lab of Agricultural Biotechnology of Ningxia;;Comparative Research of Total RNA Extraction Methods FromApocynum venetumL.[J];北方园艺;2014-11
7 HOU Si-Yu;SUN Zhao-Xia;GUO Bin;WANG Yu-Guo;LI Gui-Quan;HAN Yuan-Huai;School of Agriculture, Shanxi Agricultural University;Institute of Agricultural Biotechnology, Shanxi Agricultural University;Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture;School of Agriculture,Shanxi Agricultural University;;Cloning and Expression Analysis of Two C2H2 Transcription Factors in Soybean[J];植物生理学报;2014-05
8 ZHANG Xiao-li;DAI Hong-jun;Agricultural College,Ningxia University;;Research Progress on Extraction Method of Plant RNA[J];北方园艺;2014-08
9 Zhang Lijie;Dong Wenxuan;College of Horticulture, Shenyang Agricultural University;;Study of The Extraction Methods of Total RNA from Malus hupehensis[J];分子植物育种;2014-02
10 WANG Qi-hai;WANG Yi-na;LIU Xiao-hua;WU Wen-jiang;DING Ning;NIU Hong-bin;WANG Lin-zhong;WU Guo-liang;College of Horticulture,Henan Agricultural University;College of Food Science and Technology,Henan Agricultural University;National Center of Engineering and Technological Research for Wheat,Henan Agricultural University;;Comparative Analysis of Total RNA Extraction Methods for Leaves of Peach at Different Development Stages[J];河南农业科学;2014-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 DOU Dao Long 1,2 WANG Bing Shan 2 TANG Yi Xiong 2 WANG Zhi Xing 2 SUN Jing San 1, ( 1 Institute of Botany, Chinese Academy of Sciences, Beijing 100093; 2 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China);A Simple and Efficient Method for Extraction of High Quality RNA from Cotton[J];作物学报;2003-03
2 Yao Hongyan Zhao Shuangyi Xia Guangmin(School of Life Science Shandong University Jinan 250100);Improved Urea-LiCl Method for Extracting Total RNA from Wheat Mature Seeds[J];中国生物工程杂志;2003-04
3 SHEN WenBiao,Wang Ren,WANG YiHua,ZHENG TianQing,WAN JianMinesearch Institute,Nanjing Agricultural University,Nanjing 210095,China);A Novel Method of Extracting Total RNA from Rice Embryo Samples[J];遗传;2003-02
4 Du Xihua; Lu Hai; Gao Shumin; Li Fenglan. School of Biological Science and Biotechnology, Beijing For. Univ., 100083, P. R. China.;Method for RNA isolating from anthers of Xanthoceras sorbifolia Bunge.[J];北京林业大学学报;2003-01
5 WANG Fang1,2, ZHANG Bin2, SHAN Lei2, BI Yu-ping2, XU Ze-yong3(1. School of Life Sciences, Shandong Normal University, Jinan 250014,China; 2. Plant Biology Technology Research Center, SAAS, Jinan 250100,China; 3. Oil Crops Research Institute ,CAAS,Wuhan 430062,China);An Improved Method for RNA Isolation from Peanut Seeds[J];花生学报;2002-04
6 ZHAO Shuang yi,WU Yao rong,XIA Guang min (School of Life Sciences,Shandong University,Jinan 250100,China);Introduction of a Simple and Effective Method for Plant Totle RNA Isolation[J];遗传;2002-03
7 Wang Yucheng, Yang Chuanping, Jiang Jing(Northeast Forestry University, Harbin 150040, P. R. China);The Main Points and Principles of Isolating Total RNA from Ligneous Plant Tissues[J];东北林业大学学报;2002-02
8 ZHANG Yi,DUAN Liu sheng,ZHAI Zhi xi,HE Zhong pei(Research Center of Crop Chemical Control,China Agricultural University,Beijing 100094,China);Establishment of liquid culture method for in vitro maize root tip with filter-papers-bed and motionless[J];西北植物学报;2001-06
9 XIA Lan qin,GUO San dui (The Biotechnology Research Center of CAAS, Beijing 100081,China);A Method of RNA fast Extracing in Cotton Tissues[J];棉花学报;2000-04
10 Li Hong 1,2 Wang Xinli 1 1 Laboratory of Plant Biotechnology, Institute of Micobiology, Chinese Academy of Sciences, Bejing, 100080; 2 Department of Light Industry, Hebei Sciences and Technology University, Shijiazhuang, 050018;The Difficulties in the Isolation of RNA from Plant Tissues and their Resolving Strategies[J];生物技术通报;1999-01
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