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《Journal of Northwest A & F University(Natural Science Edition)》 2007-07
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Study on immortalization of swine umbilicus veins endothelial cells

MA Quan-li,HONG Hai-xia,ZHANG Yan-ming,XU Hao,YANG Meng,SU Zheng-yuan(College of Animal Science and Technology,Northwest A & F Univercity,Yangling,Shaanxi 712100,China)  
To establish stable vascular endothelial cell line as an ideal cell model in the research of facilitating the study on pathologic mechanism of CSFV,swine umbilical vein endothelial primary cells were transfected with the plasmid pCIneo-hTERT by liposome.Positive cells clone were selected by G418.The morphous,proliferation and growth characteristics of tansfected cell were observed and studied,including the identification of telomerase activation.The result showed that the confluent monolayer transfected cells looked like cobblestones with contact inhibition.Immunofluorescence staining showed the transfected cells Factor-Ⅷ and CD34 positive.The number of cell passage increased after transfection and the telomerase activation was positive at the 10th passage and 15th passage.The results demonstrated the transfection of hTERT gene could prolong the swine umbilical vein endothelial cell survival.
【Fund】: 国家自然科学基金项目(编号:30471290)
【CateGory Index】: Q813
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