Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Northwest A & F University(Natural Science Edition)》 2009-01
Add to Favorite Get Latest Update

Antioxidation analisis of extracts from petals of 6 tree peonies in vitro

FEN Zhi-wen,YANG Xia-guang,PAN Jian,REN Guang-hui, ZHANG Er-xi,FAN Bing-you,SHI Guo-an (Luoyang Key Laboratory of Peony Biology,College of Agriculture,Henan University of Science &Technology,Luoyang,Henan 471003,China)  
【Objective】 To extract the antioxidant compounds from peony flowers,its effect of cleaning oxygen radical was analyzed.【Method】 The extracts from 6 peonies petals were isolated by water and 50% ethanol.Its antioxidation activities were detected and evaluated by four systems of DPPH,O-·2,·OH and PUFA with controls for Vc and BHT.Water or 50% ethanol extracts of 6 peonies petals were added to the generating and detecting systems of DPPH radical,hydroxyl radical(·OH),superoxide radical(O-·2) and PUFA peroxidation of yolk lipoprotein for the observation of its competitive abilities to active reactions.【Result】 The results indicated that the water or 50% ethanol extracts from peonies petals could reduce light absorption of DPPH and ·OH initiated hydroxylation of salicylate,and inhibit O-·2 mediated light chemical reduction of nitroblue tetrazolium(NBT) effectively,and lipid peroxidation of polyunsaturated fatty from yolk lipoprotein,in which DPPH scavenging activities of 6 peonies water extracts were hinger than that of 200 μg/mL BHT,but lower than 80 μg/mL Vc.In 4 kinds of detecting systems of antioxidation,the peroxidation activity of deep colored flowers of peonies was higher than that of light colored flowers of peonies.【Conclusion】 The peony flower can be used as an antioxidation.
【Fund】: 国家自然科学基金项目(30740013);; 河南省自然科学基金项目(0611030600);; 河南科技大学大学生科研训练计划(SRTP)项目(2006059)
【CateGory Index】: S685.11
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved