Analysis of genetic diversity in cotton varieties of intercropping of apricot and cotton around Tarim Basin

ZHAO Guang-lei1,ZHU Hong-ju1,Liu Chun-jing2,CHEN Rui-ping2,CAO Chun-bao2,TAN Yong-jun2,CHEN Yao-feng1(1 College of Agriculture,Northwest A&F University,Yangling,Shaanxi 712100,China;2 Agriculture Technical Extension Centre,Luntai,Xinjiang 841600,China)  

[Objective] This study was to illustrate genetic diversity of main cotton varieties in intercropping of fruit and crop,which provided a theoretical basis for breeding and selection of cotton varieties.[Method] The randomly amplified polymorphic DNA procedure(RAPD)was used to assess the genetic diversity of 23 cotton varieties of the intercropping in the Tarim Basin in this study,and the software of NTSYS-pc version 2.10 was used to calculate similarity coefficients and construct a dendrogram.[Result] A total of 70 arbitrary primers were screened using RAPD markers with the 23 cotton species and finally 27 primers which could produce steady polymorphism were obtained.136 polymorphic bands were amplified and the polymorphic rate was 67.3%.Mean similarity among 23 cotton varieties of the intercropping was 0.665,in which more than 0.7 accounted for 26.09% and less than 0.5 was only 6.72%.Cluster analysis revealed that these 23 cotton species could be divided into 2 classes and 5 subclasses,which clarified the genetic diversity of these species.[Conclusion] There was a low level of genetic diversity among these cotton varieties of the intercropping in the Tarim Basin.

【Key Words】： intercropping of fruit and crop cotton variety RAPD analysis genetic diversity cluster analysis
【Fund】： “十一五”国家科技支撑计划项目(2007BAD36B03-3)
【CateGory Index】： S562.02

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