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《Journal of Northwest A & F University(Natural Science Edition)》 2012-08
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Effects of different collecting pollen periods and storage conditions on pollen viability of kiwifruit

CHEN Yong-an,CHEN Xin,LIU Yan-fei (College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China)  
【Objective】 Research about effects of different collecting pollen periods and storage conditions on pollen viability of kiwifruit was conducted for the production of artificial auxiliary pollination and hybrid breeding with favorable basis.【Method】 With Zhonghuaxi 401 as test materials,pollens in flowering before 3,2,1 d big budding period and flowering 6,12,24 h were collected;With soft dates kiwifruit,Qinxiong 201,Zhonghuaxi 401 and Maohua kiwifruit as test materials,pollens in big budding period were collected,and stored under normal temperature(18-26 ℃),low temperature(4 and-15 ℃).Pollen viability of kiwifruit in different collecting pollen periods and storage conditions was compared.【Result】 Kiwifruit pollen viability has only 1.92% in the flowering before 3 d;kiwifruit pollen viability has reached 48.07% and 69.11% in the flowering before 2 d and 1 d;the big budding pollen viability was the highest,reaching 82.31%.In the flowering 6 h,12 h and 24 h,pollen viability was 66.89%,46.03% and 31.40%,and they all were lower than the big budding.Four different kinds of kiwifruit fresh pollen viability was all higher,more than 70%.Under normal conditions,kiwifruit pollen can only be stored for 6-9 d,at 4 ℃ refrigerated condition pollen life would maintain 60-150 d,average storage can be as long as 90 d,and under-15 ℃ frozen conditions pollen viability can keep longer,would reach 360 d.【Conclusion】 It is better to use pollen in big budding;temperature is one of the important factors affecting kiwifruit pollen viability.The lower the temperature is,the better the results of the pollen storage is.
【Fund】: 国家财政部“以大学为依托的农业科技推广模式建设”项目(XTG2009-10);; 国家林业局“948”引进项目(2011-4-46)
【CateGory Index】: S663.4
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