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Identification of Genetic Marker Linked to Seedless Genes in Grapes Using RAPD

Wang Y J 1 Lamikanra O 2 Lu J 2 Ramming D W 3 (1 Department of Horticulture,Northwestern Agricultural University, Yangling, Shaanxi 712100, the People’s Republic of China) (2 Center for Viticultural Science, Florida A & M  
This paper reports the rapid detection of a genetic marker linked to seedless genes in segregant populations of grapes using random primers and BSA (bulked segregant analysis). Two bulked DNA samples were generated from cross combination of B72 216×B45 187. One bulk contains mixed DNA samples of 9 individuals that are identical for the seedlessness trait in phenotype, and another bulk are those of 9 individuals of the same family with the seeded trait, but arbitrary at all unlinked regions. The bulks were first screened for polymorphism using a set of 100 arbitrary 10 mers primers. Only one polymorphism in the seedless trait pool distinguished two bulks by primer UBC 269. The size of this polymorphism is 500 bp. The polymorphism was further featured in some hybrids and the pollen parent B45 187 as well as initial donors of seedless genes, Thompson seedless, and Perlette, when they were used as the templates for amplified by UBC 269. Based on the presence or absence of this polymorphism, segregation that can be mapped in the population occurs. Our results suggested that the seedlessness of grapes is controlled by multigenes, including major and minor genes; The marker, UBC 269 500 obtained in the study, was linked to one of the seedless genes in grapes. and it appears to be related to a major seedless gene.
【CateGory Index】: S663.103.2
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