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Biotransformation of 24-methylenecholesterol and sitosterol by yeast mutant GL-7

Guo Dean; Hu Jun; Cui Yajun; Guo Hongzhu; Zheng Junhua and Nes WD(School of Pharmaceutical Sciences,Beijing Medical University, Beijing 100083)  
Incubations of 24-methylenecholesterol and sitosterol with yeast mutant GL-7 afforded their corresponding 22-desaturated products under the catalysis of sterol △22(33)-desaturase. The products were identified to be brassicasterol and stigmasterol respectively based on their chromatographic and spectral properties as well as by comparison with the authentic samples.
【CateGory Index】: R914
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1 LI Chun-yuan1,SHAO Chang-lun2,DING Wei-jia3,*(1.College of Science,South China Agricultural university Guangzhou 510642,China;2.School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China);Chemical constituents of the mycelia of an entomogenous fungus (No.GIM3.94) from Helicoverpa armigera[J];Journal of Zhoukou Normal University;2008-02
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