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《Northwest Pharmaceutical Journal》 2018-02
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Extraction of emodin from Reynoutria japonica Houtt.by semi-bionic enzyme method

XIAN Liang;GUO Zengjun;QIN Yuanrui;ZHANG Hui;School of Pharmacy,Xi′an Jiaotong University;  
Objective To study the suitable semi-bionic enzyme extraction method of emodin from Reynoutria japonica Houtt..Methods The content of the emodin in all the extracts was determined by HPLC.The cellulase was selected and the semi-bionic cellulase extractions were optimized by orthogonal experiment.Then the semi-bionic enzyme extraction method was compared with ultrasonic extraction,microwave extraction and ethanol extraction methods.Results The method of semi-bionic enzyme extraction is more efficient than the others.The preferred process of the orthogonal experiment was determined as follows:extraction temperature at 70 ℃,solid-liquid ratio of 1∶18 and extraction time for 2 h.Conclusion Semi-bionic enzyme extraction is an economical and effective method for emodin,which can be used in the extraction process of Reynoutria japonica Houtt..
【Fund】: 国家自然科学基金项目(编号:81172957);; 陕西省自然科学基金项目(编号:2015JQ2048 2016JM8075)
【CateGory Index】: R284.2
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