Evaluation of Soybean Germplasm with Random Amplification Polymorphic DNA(RAPD)Markers
Qiu Lijuan Randall L. Nelson Lila O. VodkinInstitute of Crop Germplasm Resources, CAAS, Beijing 100081, China; USDA Soybean Germplasm Collection, Urbana, 1L 61801, USA, Department of Crop Science, University of Illinois, Urbana, IL 61801, USA
Fifty-seven Chinese ancestral lines and their derived cultivars and eighteen US ancestral lines were used to evaluate genetic diversities by RAPD markers between or within Chinese and US lines. Fifty 10-mer random primers were used for PCR reactions. The total of 246 distinguishable PCR products were detected by agarose gel electrophoresis among 75 accessions, 82. 3% of random primers were polymorphic and 54. 4% of total produts were different at least between two lines, which can be used as RAPD markers to identify soybean germplasm resources. Thirty-two soybean accessions had their special PCR banding patterns. Each of those amplified bands was taken as one of the variant which was record as 1 for present and 0 for absent. Comparison among all possible a pair of data were calculated to produce non-similarity matrix and to build up a pedigree tree. The cluster analysis indicated that not only Chinese ancestral lines were different from US ancestral lines, but northern soybean accesstions were different from southern ones within two countries also. The obvious geographic differences provide possibilities to broaden genetic base of Chinese soybean cultivars by selecting germplasm from various regions within or between countries.