Analysis of RAPD Fingerprinting on Shortseasoned Cotton Cultivars in China
Wang Xinyu Guo Wangzhen Zhang Tianzhen Pan Jiaju(Cotton Genetics & Breeding Research Lab, Nanjing Agricultural University,Nanjing 210095)
RAPD(Random Amplified Polymorphic DNA) technique was used to detect the genetic diversity of 25 short-seasoned cotton cultivars in China. The results indicated that 25 short-seasoned cotton cultivars could be divided into four subgroups based on cluster and similarity analysis of RAPD fingerprinting of each cultivar amplified with 18 random primers. Most of the tested cultivars were consistent with their pedigrees, i. e, most of short-seasoned cotton cultivars were developed from King cultivar introduced from USA. It is further testified that the genetic basis of popularized short-seasoned cotton cultivars in China is rather narrow and it is essen-sial to find some new germplasms for the development of new cultivars. The study is also valuable in the choice of parents for the development of cotton cultivars and hybrids.