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《Acta Agronomica Sinica》 2000-03
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RFLP Mapping of a Male Sterile Gene ( ms 30 ) in Maize

LIANG Ye Hong ZHOU Hong Sheng JIANG Wan Ru[WT6BX](Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing, 100081)[KH+3mmD][WT5HZ]  
A sibling population SIB 5 and backcross population BC 1 were applied to map a male sterile gene ms 30 which was early located on chromosome 4 of maize by B A system. 18 probes from maize chromosome 4 were used, and BSA was practised to screen on RFLPs. By using JoinMap software, linkage as well as genetic distance between ms 30 and marker loci were obtained. The main result as follows:analysis on SIB 5 population showed that ms 30 was linked with umc15a and umc66a on maize 4L, the recombination value was 5.9 % and 14.8 %, respectively. In the BC 1 population, umc19, umc15a, bn17.65 and csu178a were found linked to ms 30 , the genetic distance was umc19 14cM ms 30 4.2 cM umc15a 1.4 cM bn 17.65 3.4 cM csu178a.
【Fund】: 国家自然科学基金课题! (批准号 :39480 0 13)
【CateGory Index】: S513
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